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Key=description, value=Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
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Key=contents, value=Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Key=synonyms, value=Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19;KEAP1;INRF2, KIAA0132, KLHL19;
Key=application_details, value=WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
Key=subcellular_localization, value=Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Key=tissue_specificity, value=Broadly expressed, with highest levels in skeletal muscle.
Key=protein_function, value=Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
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Key=purification, value=The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
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Anti-Keap1 Antibody

KEAP1 antibody

Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00514-2
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Keap1 Antibody

View all KEAP1 Antibodies

SKU/Catalog Number

A00514-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Keap1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00514-2)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00514-2 is reactive to KEAP1 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

69666 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00514-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000

Validation Images & Assay Conditions

Gene/Protein Information For KEAP1 (Source: Uniprot.org, NCBI)

Gene Name

KEAP1

Full Name

Kelch-like ECH-associated protein 1

Weight

69666 MW

Superfamily

KEAP1 family

Alternative Names

Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19;KEAP1;INRF2, KIAA0132, KLHL19; KEAP1 INrf2, KLHL19 kelch like ECH associated protein 1 kelch-like ECH-associated protein 1|KEAP1 delta C|cytosolic inhibitor of Nrf2|kelch-like family member 19|kelch-like protein 19

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KEAP1, check out the KEAP1 Infographic

KEAP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KEAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00514-2 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
A00514-2 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200

Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling

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Order DetailsPrice
A00514-2-100ul

100ul

$380

Free Secondary Antibody

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A00514-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$380.00

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