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Key=store_id Key=entity_id, value=88814 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A00514-2 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2020-03-11 06:19:29 Key=updated_at, value=2024-10-19 05:12:10 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-Keap1 Antibody Key=cj_search_index Key=publication_count, value=2 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1375 Key=yoast_content_score, value=60 Key=price, value=380.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-Keap1 Antibody A00514-2 Key=meta_description, value=Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. Key=image, value=/a/0/a00505-western-blotting-analysis-1.jpg Key=small_image, value=/a/0/a00505-western-blotting-analysis-1.jpg Key=thumbnail, value=/a/0/a00505-western-blotting-analysis-1.jpg Key=url_key, value=anti-keap1-antibody-a00514-2-boster Key=url_path, value=anti-nrl-antibody-a00505-boster Key=options_container, value=Block after Info Column Key=image_label, value=Western blot analysis of extracts from 293 cells, using NRL antibody A00505. Key=small_image_label, value=Western blot analysis of extracts from 293 cells, using NRL antibody A00505. Key=thumbnail_label, value=Western blot analysis of extracts from 293 cells, using NRL antibody A00505. Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100ul Key=protein_name, value=Kelch-like ECH-associated protein 1 Key=gene_full_name, value=Kelch-like ECH-associated protein 1 Key=uniprot_id, value=Q14145 Key=sensitivity Key=applications, value=ELISA, IHC, WB Key=reactivity, value=Human, Mouse, Rat Key=form, value=Liquid Key=concentration, value=1 mg/ml Key=predicted_reactivity Key=source_company, value=Huafeng (order through China) HF39708, cost 100ul 650RMB Key=isotype, value=IgG Key=molecular_weight, value=69666 MW Key=sample_size_available, value=5ul, free with shipping charge Key=related_product_skus, value=A00505-1 Key=product_category, value=Primary Antibodies Key=research_category, value=Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Neuroscience, Sensory System, Transcription, Transcription Factors, Visual System Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a00478-anti-fibroblast-growth-factor-23-fgf23-antibody-wb-testing-1_1.jpg Key=rating_value, value=100 Key=review_count, value=5 Key=mp_exclude_sitemap, value=No Key=yoast_facebook_image, value=/a/0/a00446y341-wb-1.jpg Key=yoast_twitter_image, value=/a/0/a00446y341-wb-1.jpg Key=cj_related_products, value=Abnormal Degeneration:EK2113|A06300^Defense Response:PB9762|A01042|A05704-2 Key=description, value=Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Key=short_description, value=Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Key=meta_keyword, value=Anti-Keap1 Antibody Key=contents, value=Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Key=background Key=gene_name, value=KEAP1 Key=synonyms, value=Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19;KEAP1;INRF2, KIAA0132, KLHL19;
Key=application_details, value=WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000 Key=subcellular_localization, value=Cytoplasm. Nucleus. Shuttles between
cytoplasm and nucleus.
Key=tissue_specificity, value=Broadly expressed, with highest levels in
skeletal muscle. Key=protein_function, value=Acts as a substrate adapter protein for the E3 ubiquitin
ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for
ubiquitination and degradation by the proteasome, thus resulting
in the suppression of its transcriptional activity and the
repression of antioxidant response element-mediated detoxifying
enzyme gene expression. Retains NFE2L2/NRF2 and may also retain
BPTF in the cytosol. Targets PGAM5 for ubiquitination and
degradation by the proteasome. Key=application_notes, value=This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract. Key=competitor_equivalent_skus, value=sc 166087|sc 10972|sc 10971|sc 33183|sc 374277|sc 398046|sc 10972 X|sc 10971 X|sc 33183 X|sc 166087 X|sc 374277 X|sc 398046 X Key=immunogen, value=The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460 Key=purification, value=The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-Keap1 Antibody
KEAP1 antibody
Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).
Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Keap1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00514-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00514-2 is reactive to KEAP1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69666 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin
Click image to see more details
Figure 3. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin
Click image to see more details
Figure 4. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain
Click image to see more details
Figure 5. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain
Click image to see more details
Figure 1. Western blotting validation for Anti-Keap1 Antibody A00514-2
Western Blot (WB) analysis of K562 cells using Keap1 polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Gene/Protein Information For KEAP1 (Source: Uniprot.org, NCBI)
Gene Name
KEAP1
Full Name
Kelch-like ECH-associated protein 1
Weight
69666 MW
Superfamily
KEAP1 family
Alternative Names
Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19;KEAP1;INRF2, KIAA0132, KLHL19;
KEAP1 INrf2, KLHL19 kelch like ECH associated protein 1 kelch-like ECH-associated protein 1|KEAP1 delta C|cytosolic inhibitor of Nrf2|kelch-like family member 19|kelch-like protein 19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on KEAP1, check out the KEAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KEAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print Species: Mouse A00514-2 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$380.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.