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Product Info Summary
| SKU: | A00514-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Keap1 Antibody
SKU/Catalog Number
A00514-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Keap1 Antibody catalog # A00514-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Keap1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00514-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00514-2 is reactive to KEAP1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69666 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00514-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin
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Figure 3. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin
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Figure 4. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain
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Figure 5. Immunohistochemistry validation of KEAP1 using Anti-Keap1 Antibody (A00514-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain
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Figure 1. Western blotting validation for Anti-Keap1 Antibody A00514-2
Western Blot (WB) analysis of K562 cells using Keap1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For KEAP1 (Source: Uniprot.org, NCBI)
Gene Name
KEAP1
Full Name
Kelch-like ECH-associated protein 1
Weight
69666 MW
Superfamily
KEAP1 family
Alternative Names
Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19;KEAP1;INRF2, KIAA0132, KLHL19; KEAP1 INrf2, KLHL19 kelch like ECH associated protein 1 kelch-like ECH-associated protein 1|KEAP1 delta C|cytosolic inhibitor of Nrf2|kelch-like family member 19|kelch-like protein 19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KEAP1, check out the KEAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KEAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Keap1 Antibody (A00514-2)
Hello CJ!
A00514-2 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
A00514-2 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200
Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling
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