Anti-JMJD3 KDM6B Antibody

Product Name

Anti-JMJD3 KDM6B Antibody

View all Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibodies

SKU/Catalog Number

A01309

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-JMJD3 KDM6B Antibody (Catalog # A01309). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

JMJD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-JMJD3 KDM6B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01309)

Host

Rabbit

Contents

JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-JMJD3 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3. The immunogen is located within amino acids 1370-1420 of JMJD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody is specific for JMJD3 and will not recognize other JMJD proteins.

Reactive Species

A01309 is reactive to KDM6B in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

176632 MW

Background of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3

The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01309 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.25-4 μg/mL; ICC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For KDM6B (Source: Uniprot.org, NCBI)

Alternative Names

JMJD3, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3 KDM6B JMJD3, NEDCFSA lysine demethylase 6B lysine-specific demethylase 6B|[histone H3]-trimethyl-L-lysine(27) demethylase 6B|jmjC domain-containing protein 3|jumonji domain containing 3, histone lysine demethylase|jumonji domain-containing protein 3|lysine (K)-specific demethylase 6B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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