Product Info Summary
SKU: | A06605 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-KCNJ4/Kir2.3 Antibody
SKU/Catalog Number
A06605
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-KCNJ4/Kir2.3 Antibody (Catalog # A06605). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNJ4/Kir2.3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06605)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Standard Protein
Synthesized peptide derived from internal of human KCNJ4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06605 is reactive to KCNJ4 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
49500 MW
Background of KIR2.3
Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium By similarity.
Perier F., Proc. Natl. Acad. Sci. U.S.A. 91:6240-6244(1994).
Tang W., FEBS Lett. 348:239-243(1994).
Makhina E.N., J. Biol. Chem. 269:20468-20474(1994).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06605 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
![a06605 western blotting analysis 1 a06605 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a06605-western-blotting-analysis-1.jpg)
Click image to see more details
Western blot analysis of extracts from 293 cells and NIH/3T3 cells, using KCNJ4 antibody A06605.
Protein Target Info & Infographic
Gene/Protein Information For KCNJ4 (Source: Uniprot.org, NCBI)
Gene Name
KCNJ4
Full Name
Inward rectifier potassium channel 4
Weight
49500 MW
Superfamily
inward rectifier-type potassium channel (TC 1.A.2.1) family
Alternative Names
hippocampal inward rectifier potassium channel; Hippocampal inward rectifier; HIRhIRK2; HIRK2; HRK1MGC142066; Inward rectifier K(+) channel Kir2.3; inward rectifier K+ channel Kir2.3; inward rectifier potassium channel 4; IRK-3; IRK3MGC142068; Kir2.3; Potassium channel, inwardly rectifying subfamily J member 4; potassium inwardly-rectifying channel, subfamily J, member 4 KCNJ4 HIR, HIRK2, HRK1, IRK-3, IRK3, Kir2.3 potassium inwardly rectifying channel subfamily J member 4 inward rectifier potassium channel 4|hippocampal inward rectifier potassium channel|inward rectifier K(+) channel Kir2.3|potassium channel, inwardly rectifying subfamily J, member 4|potassium voltage-gated channel subfamily J member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNJ4, check out the KCNJ4 Infographic
![KCNJ4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNJ4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNJ4/Kir2.3 Antibody (A06605)
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No publications found for A06605
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Customer Q&As
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3 Customer Q&As for Anti-KCNJ4/Kir2.3 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endothelial cell using anti-KCNJ4/Kir2.3 antibody A06605. Let me know if you need anything else.
V. Evans
Verified customer
Asked: 2016-05-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-04
Question
We are interested in to test anti-KCNJ4/Kir2.3 antibody A06605 on mouse endothelial cell for research purposes, then I may be interested in using anti-KCNJ4/Kir2.3 antibody A06605 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Johnson
Verified customer
Asked: 2014-06-05
Answer
The products we sell, including anti-KCNJ4/Kir2.3 antibody A06605, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-06-05
Question
Here is the WB image, lot number and protocol we used for endothelial cell using anti-KCNJ4/Kir2.3 antibody A06605. Please let me know if you require anything else.
D. Lewis
Verified customer
Asked: 2013-08-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-05