Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3)

KAP1 antibody

Boster Bio Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) catalog # M00409-2. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: M00409-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3)

View all KAP1 Antibodies

SKU/Catalog Number

M00409-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) catalog # M00409-2. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00409-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

9E3

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00409-2 is reactive to TRIM28 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

100 kDa

Calculated molecular weight

88.55kDa

Background of KAP1

Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00409-2 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human Hela whole cell,, human PC-3 whole cell,, human HEK293 whole cell,, human A549 whole cell,, human Jurkat whole cell,, human THP-1 whole cell,, rat PC-12 whole cell,, mouse NIH/3T3 whole cell
IHC: human mammary cancer tissue, human rectal cancer tissue, rat intestine tissue
IHC-F: human placenta tissue
ICC/IF: U20S cell
IF: human rectal cancer tissue
FCM: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For TRIM28 (Source: Uniprot.org, NCBI)

Gene Name

TRIM28

Full Name

Transcription intermediary factor 1-beta

Weight

88.55kDa

Superfamily

TRIM/RBCC family

Alternative Names

E3 SUMO protein ligase TRIM28 antibody; E3 SUMO-protein ligase TRIM28 antibody; FLJ29029 antibody; KAP 1 antibody; KAP-1 antibody; KRAB associated protein 1 antibody; KRAB interacting protein 1 antibody; KRAB-associated protein 1 antibody; KRAB-interacting protein 1 antibody; KRIP 1 antibody; KRIP-1 antibody; KRIP1 antibody; Nuclear corepressor KAP 1 antibody; Nuclear corepressor KAP-1 antibody; RING finger protein 96 antibody; RNF96 antibody; TF1B antibody; TIF1 beta antibody; TIF1-beta antibody; TIF1B antibody; TIF1B_HUMAN antibody; Transcription intermediary factor 1 beta antibody; Transcription intermediary factor 1-beta antibody; Trim28 antibody; Tripartite motif containing 28 antibody; tripartite motif containing protein 28 antibody; Tripartite motif-containing protein 28 antibody TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B tripartite motif containing 28 transcription intermediary factor 1-beta|E3 SUMO-protein ligase TRIM28|KAP-1|KRAB [Kruppel-associated box domain]-associated protein 1|KRAB-interacting protein 1|KRIP-1|RING finger protein 96|RING-type E3 ubiquitin transferase TIF1-beta|TIF1-beta|nuclear corepressor KAP-1|protein phosphatase 1, regulatory subunit 157|transcriptional intermediary factor 1-beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRIM28, check out the TRIM28 Infographic

TRIM28 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRIM28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00409-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hao Y,Bai S,Peng J,Hong R,Ding J,Li Z,Guan Y.TRIM27-mediated ubiquitination of PPARγ promotes glutamate-induced cell apoptosis and inflammation.Exp Cell Res.2020 Dec 29:112437.doi:10.1016/j.yexcr.2020. 112437.Epub ahead of print.PMID:33385414.
Species: Human
M00409-2 usage in article: APP:WB, SAMPLE:HT22 CELL, DILUTION:NA

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Order DetailsPrice
M00409-2

100μg

$370
M00409-2-10ug

10μg sample (liquid)

$99
M00409-2-Biotin

100 μg Biotin conjugated

$570
M00409-2-Cy3

100 μg Cy3 conjugated

$570
M00409-2-Dylight488

100 μg Dylight488 conjugated

$570
M00409-2-Dylight550

100 μg Dylight550 conjugated

$570
M00409-2-Dylight594

100 μg Dylight594 conjugated

$570
M00409-2-FITC

100 μg FITC conjugated

$570
M00409-2-HRP

100 μg HRP conjugated

$570
M00409-2-APC

100 μg APC conjugated

$670
M00409-2-PE

100 μg PE conjugated

$670
M00409-2-iFluor647

100 μg iFluor647 conjugated

$670
M00409-2-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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