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Product Info Summary
| SKU: | PB10005 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Kallikrein 8/KLK8 Antibody Picoband®
View all Kallikrein 8/Neuropsin Antibodies
SKU/Catalog Number
PB10005
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 8/KLK8 Antibody Picoband® catalog # PB10005. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 8/KLK8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10005)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Kallikrein 8 recombinant protein (Position: V33-G260). Human Kallikrein 8 shares 74% and 73.1% amino acid (aa) sequence identity with mouse and rat Kallikrein 8, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10005 is reactive to KLK8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
28048 MW
Background of Kallikrein 8/Neuropsin
Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10005 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Kallikrein 8 using anti-Kallikrein 8 antibody (PB10005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 8 antigen affinity purified polyclonal antibody (Catalog # PB10005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 8 at approximately 28 kDa. The expected band size for Kallikrein 8 is at 28 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KLK8 (Source: Uniprot.org, NCBI)
Gene Name
KLK8
Full Name
Kallikrein-8
Weight
28048 MW
Superfamily
peptidase S1 family
Alternative Names
Kallikrein-8;hK8;3.4.21.118;Neuropsin;NP;Ovasin;Serine protease 19;Serine protease TADG-14;Tumor-associated differentially expressed gene 14 protein;KLK8;NRPN, PRSS19, TADG14;UNQ283/PRO322; KLK8 HNP, NP, NRPN, PRSS19, TADG14 kallikrein related peptidase 8 kallikrein-8|ovasin|serine protease 19|serine protease TADG-14|tumor-associated differentially expressed gene 14 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK8, check out the KLK8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 8/KLK8 Antibody Picoband® (PB10005)
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1 Customer Q&As for Anti-Kallikrein 8/KLK8 Antibody Picoband®
Question
We are currently using anti-Kallikrein 8/KLK8 antibody PB10005 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
C. Krishna
Verified customer
Asked: 2018-02-14
Answer
The anti-Kallikrein 8/KLK8 antibody (PB10005) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-14
