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Product Info Summary
| SKU: | PA1439 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®
View all Carbonic Anhydrase III/CA3 Antibodies
SKU/Catalog Number
PA1439
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Carbonic Anhydrase III/CA3 Antibody catalog # PA1439. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1439)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CA3, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1439 is reactive to CA3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
29557 MW
Background of Carbonic Anhydrase III/CA3
Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. CA3 is found in high concentration in muscle. CA3 is used in conjunction with creatine kinase detection of the carrier state for Duchenne muscular dystrophy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1439 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
WB: SMMC Cell, HELA Cell, SW620 Cell, SCG Cell
IHC: Rat Skeletal Muscle Tissue
Validation Images & Assay Conditions
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Anti-CA3 antibody, PA1439, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: SCG Cell Lysate
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Anti-CA3 antibody, PA1439, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Protein Target Info & Infographic
Gene/Protein Information For CA3 (Source: Uniprot.org, NCBI)
Gene Name
CA3
Full Name
Carbonic anhydrase 3
Weight
29557 MW
Superfamily
alpha-carbonic anhydrase family
Alternative Names
Carbonic anhydrase 3;4.2.1.1;Carbonate dehydratase III;Carbonic anhydrase III;CA-III;CA3; CA3 CAIII, Car3 carbonic anhydrase 3 carbonic anhydrase 3|CA-III|HEL-S-167mP|carbonate dehydratase III|carbonic anhydrase III, muscle specific|carbonic anhydrase IIII|epididymis secretory sperm binding protein Li 167mP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CA3, check out the CA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® (PA1439)
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