Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®

Product Name

Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®

View all Carbonic Anhydrase III/CA3 Antibodies

SKU/Catalog Number

PA1439

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Carbonic Anhydrase III/CA3 Antibody catalog # PA1439. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1439)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CA3, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1439 is reactive to CA3 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

23 kDa

Calculated molecular weight

29557 MW

Background of Carbonic Anhydrase III/CA3

Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. CA3 is found in high concentration in muscle. CA3 is used in conjunction with creatine kinase detection of the carrier state for Duchenne muscular dystrophy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1439 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat

Positive Control

WB: SMMC Cell, HELA Cell, SW620 Cell, SCG Cell
IHC: Rat Skeletal Muscle Tissue

Validation Images & Assay Conditions

Gene/Protein Information For CA3 (Source: Uniprot.org, NCBI)

Alternative Names

Carbonic anhydrase 3;4.2.1.1;Carbonate dehydratase III;Carbonic anhydrase III;CA-III;CA3; CA3 CAIII, Car3 carbonic anhydrase 3 carbonic anhydrase 3|CA-III|HEL-S-167mP|carbonate dehydratase III|carbonic anhydrase III, muscle specific|carbonic anhydrase IIII|epididymis secretory sperm binding protein Li 167mP

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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