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Product Info Summary
| SKU: | PB10049 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®
View all Carbonic Anhydrase III/CA3 Antibodies
SKU/Catalog Number
PB10049
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® catalog # PB10049. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10049)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CA3 recombinant protein (Position: D14-E235). Human CA3 shares 92.3% and 91.4% amino acid (aa) sequence identity with mouse and rat CA3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10049 is reactive to CA3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
29557 MW
Background of Carbonic Anhydrase III/CA3
Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10049 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat cardiac muscle tissue, mouse cardiac muscle tissue
IHC: mouse skeletal muscle tissue, rat skeletal muscle tissue, human osteosarcoma tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CA3 using anti-CA3 antibody (PB10049).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat cardiac muscle tissue lysates,
Lane 2: mouse cardiac muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA3 antigen affinity purified polyclonal antibody (Catalog # PB10049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA3 at approximately 29 kDa. The expected band size for CA3 is at 30 kDa.
Click image to see more details
Figure 2. IHC analysis of CA3 using anti-CA3 antibody (PB10049).
CA3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CA3 Antibody (PB10049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CA3 using anti-CA3 antibody (PB10049).
CA3 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CA3 Antibody (PB10049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CA3 using anti-CA3 antibody (PB10049).
CA3 was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CA3 Antibody (PB10049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CA3 (Source: Uniprot.org, NCBI)
Gene Name
CA3
Full Name
Carbonic anhydrase 3
Weight
29557 MW
Superfamily
alpha-carbonic anhydrase family
Alternative Names
Carbonic anhydrase 3;4.2.1.1;Carbonate dehydratase III;Carbonic anhydrase III;CA-III;CA3; CA3 CAIII, Car3 carbonic anhydrase 3 carbonic anhydrase 3|CA-III|HEL-S-167mP|carbonate dehydratase III|carbonic anhydrase III, muscle specific|carbonic anhydrase IIII|epididymis secretory sperm binding protein Li 167mP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CA3, check out the CA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® (PB10049)
Hello CJ!
PB10049 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lan F, Yue X, Han L, Shi Z, Yang Y, Pu P, Yao Z, Kang C. Int J Oncol. 2012 Feb;40(2):519-26. Doi: 10.3892/Ijo.2011.1215. Epub 2011 Sep 28. Genome-Wide Identification Of Tcf7L2/Tcf4 Target Mirnas Reveals A Role For Mir-21 In Wnt-Driven Epithelial C...
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Customer Q&As
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6 Customer Q&As for Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Carbonic Anhydrase III/CA3 antibody (PB10049)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We do provide the blocking peptide for product anti-Carbonic Anhydrase III/CA3 antibody (PB10049). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-10
Question
I see that the anti-Carbonic Anhydrase III/CA3 antibody PB10049 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-23
Question
I was wanting to use your anti-Carbonic Anhydrase III/CA3 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can see on the product datasheet, PB10049 anti-Carbonic Anhydrase III/CA3 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-25
Question
We are currently using anti-Carbonic Anhydrase III/CA3 antibody PB10049 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
J. Brown
Verified customer
Asked: 2017-02-16
Answer
The anti-Carbonic Anhydrase III/CA3 antibody (PB10049) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Carbonic Anhydrase III/CA3 antibody PB10049. Let me know if you need anything else.
T. Thomas
Verified customer
Asked: 2013-05-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-05-29
Question
Does anti-Carbonic Anhydrase III/CA3 antibody PB10049 work for WB with liver?
K. Parker
Verified customer
Asked: 2013-03-15
Answer
According to the expression profile of liver, CA3 is highly expressed in liver. So, it is likely that anti-Carbonic Anhydrase III/CA3 antibody PB10049 will work for WB with liver.
Boster Scientific Support
Answered: 2013-03-15
