Product Info Summary
SKU: | M07442-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-5T4 TPBG Rabbit Monoclonal Antibody
SKU/Catalog Number
M07442-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-5T4 TPBG Rabbit Monoclonal Antibody catalog # M07442-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-5T4 TPBG Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07442-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CDA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 5T4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07442-1 is reactive to TPBG in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
46032 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07442-1 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: MCF-7 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 5T4 expression in MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TPBG (Source: Uniprot.org, NCBI)
Gene Name
TPBG
Full Name
Trophoblast glycoprotein
Weight
46032 MW
Alternative Names
Trophoblast glycoprotein;5T4 oncofetal antigen;5T4 oncofetal trophoblast glycoprotein;5T4 oncotrophoblast glycoprotein;M6P1;Wnt-activated inhibitory factor 1;WAIF1;TPBG;5T4; TPBG 5T4, 5T4AG, M6P1, WAIF1 trophoblast glycoprotein trophoblast glycoprotein|5T4 oncofetal |5T4 oncofetal trophoblast glycoprotein|5T4 oncotrophoblast glycoprotein|wnt-activated inhibitory factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPBG, check out the TPBG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPBG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-5T4 TPBG Rabbit Monoclonal Antibody (M07442-1)
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No publications found for M07442-1
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1 Customer Q&As for Anti-5T4 TPBG Rabbit Monoclonal Antibody
Question
We are currently using anti-5T4 Rabbit Monoclonal antibody M07442-1 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
A. Evans
Verified customer
Asked: 2015-03-31
Answer
The anti-5T4 Rabbit Monoclonal antibody (M07442-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-03-31