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Product Info Summary
| SKU: | M02969-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Islet1 Monoclonal Antibody
SKU/Catalog Number
M02969-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Islet1 Monoclonal Antibody catalog # M02969-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Islet1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02969-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFIA-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Islet1 ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02969-1 is reactive to ISL1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
39036 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02969-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Islet1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ISL1 (Source: Uniprot.org, NCBI)
Gene Name
ISL1
Full Name
Insulin gene enhancer protein ISL-1
Weight
39036 MW
Alternative Names
Insulin gene enhancer protein ISL-1;Islet-1;ISL1; ISL1 ISLET1, Isl-1 ISL LIM homeobox 1 insulin gene enhancer protein ISL-1|islet-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ISL1, check out the ISL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ISL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Islet1 Monoclonal Antibody (M02969-1)
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No publications found for M02969-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Islet1 Monoclonal Antibody
Question
We were satisfied with the WB result of your anti-Islet1 Monoclonal antibody. However we have observed positive staining in testis nucleus. using this antibody. Is that expected? Could you tell me where is ISL1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
According to literature, testis does express ISL1. Generally ISL1 expresses in nucleus. Regarding which tissues have ISL1 expression, here are a few articles citing expression in various tissues:
Pancreatic islet, Pubmed ID: 7912209
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-30
Question
I see that the anti-Islet1 Monoclonal antibody M02969-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-31
Question
We have observed staining in human platelet. Do you have any suggestions? Is anti-Islet1 Monoclonal antibody supposed to stain platelet positively?
M. Wu
Verified customer
Asked: 2019-12-10
Answer
Based on literature platelet does express ISL1. Based on Uniprot.org, ISL1 is expressed in forebrain, pancreatic islet, testis, platelet, among other tissues. Regarding which tissues have ISL1 expression, here are a few articles citing expression in various tissues:
Pancreatic islet, Pubmed ID: 7912209
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-10
Question
My question regards to test anti-Islet1 Monoclonal antibody M02969-1 on human platelet for research purposes, then I may be interested in using anti-Islet1 Monoclonal antibody M02969-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The products we sell, including anti-Islet1 Monoclonal antibody M02969-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-08
Question
I have a question about product M02969-1, anti-Islet1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02969-1 anti-Islet1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-09
Question
Will anti-Islet1 Monoclonal antibody M02969-1 work on goat IP with forebrain?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
Our lab technicians have not validated anti-Islet1 Monoclonal antibody M02969-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Islet1 Monoclonal antibody M02969-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat forebrain in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-01
Question
Is this M02969-1 anti-Islet1 Monoclonal antibody reactive to the isotypes of ISL1?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The immunogen of M02969-1 anti-Islet1 Monoclonal antibody is A synthesized peptide derived from human Islet1 ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-15
Question
Will M02969-1 anti-Islet1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
You can see on the product datasheet, M02969-1 anti-Islet1 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-14
Question
Will anti-Islet1 Monoclonal antibody M02969-1 work for IP with platelet?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
According to the expression profile of platelet, ISL1 is highly expressed in platelet. So, it is likely that anti-Islet1 Monoclonal antibody M02969-1 will work for IP with platelet.
Boster Scientific Support
Answered: 2018-11-26
Question
We ordered your anti-Islet1 Monoclonal antibody for WB on pancreatic islet in a previous experiment. I am using human, and We intend to use the antibody for IF next. I am interested in examining pancreatic islet as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
I looked at the website and datasheets of our anti-Islet1 Monoclonal antibody and I see that M02969-1 has been tested on human in both WB and IF. Thus M02969-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-24
Question
I was wanting to use your anti-Islet1 Monoclonal antibody for IP for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
As indicated on the product datasheet, M02969-1 anti-Islet1 Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-31
Question
We are currently using anti-Islet1 Monoclonal antibody M02969-1 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
The anti-Islet1 Monoclonal antibody (M02969-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-19
Question
Please see the WB image, lot number and protocol we used for platelet using anti-Islet1 Monoclonal antibody M02969-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Islet1 Monoclonal antibody M02969-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-16
Question
Is a blocking peptide available for product anti-Islet1 Monoclonal antibody (M02969-1)?
A. Li
Verified customer
Asked: 2016-12-08
Answer
We do provide the blocking peptide for product anti-Islet1 Monoclonal antibody (M02969-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-12-08
Question
Do you have a BSA free version of anti-Islet1 Monoclonal antibody M02969-1 available?
J. Miller
Verified customer
Asked: 2016-03-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Islet1 Monoclonal antibody M02969-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-03-14
