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Product Info Summary
| SKU: | M00165-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IRF3 Mouse Monoclonal Antibody [Clone ID: OTI5D2]
SKU/Catalog Number
M00165-1
Size
100 µl
Description
Boster Bio Anti-IRF3 mouse monoclonal antibody, clone OTI5D2 (formerly 5D2). Catalog# M00165-1. Tested in IHC, WB. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF3 Mouse Monoclonal Antibody [Clone ID: OTI5D2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00165-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5D2
Isotype
IgG2b
Immunogen
Full-length protein expressed in 293T cell transfected with human IRF3 expression vector
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00165-1 is reactive to IRF3 in Human, Monkey
Reconstitution
Observed Molecular Weight
47.2 kDa
Calculated molecular weight
47.219kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00165-1 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-IRF3monoclonal antibody.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and IRF3-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-IRF3 monoclonal antibody M00165-1 (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
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Western blot analysis of extracts (35ug) from 4 different cell lines and human plasma by using anti-IRF3 monoclonal antibody (1:500).
Protein Target Info & Infographic
Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)
Gene Name
IRF3
Full Name
Interferon regulatory factor 3
Weight
47.219kDa
Superfamily
IRF family
Alternative Names
interferon regulatory factor 3; IRF3; IRF-3 IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF3, check out the IRF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF3 Mouse Monoclonal Antibody [Clone ID: OTI5D2] (M00165-1)
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