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Product Info Summary
| SKU: | A00165 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Interferon regulatory factor 3 IRF3 Antibody
SKU/Catalog Number
A00165
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Interferon regulatory factor 3 IRF3 Antibody (Catalog # A00165). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
IRF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Interferon regulatory factor 3 IRF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00165)
Host
Rabbit
Contents
IRF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3. The immunogen is located within amino acids 330 - 380 of IRF3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of IRF3 are known to exist.
Reactive Species
A00165 is reactive to IRF3 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
47219 MW
Background of IRF3
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00165 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IRF3 in Ramos whole cell lysate with IRF3 antibody at (A) 1
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Immunohistochemical staining of mouse kidney using IRF3 antibody at 2 μg/mL.
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Immunofluorescence of IRF3 in Mouse Kidney cells with IRF3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)
Gene Name
IRF3
Full Name
Interferon regulatory factor 3
Weight
47219 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 3, IRF-3 IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF3, check out the IRF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon regulatory factor 3 IRF3 Antibody (A00165)
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