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Product Info Summary
| SKU: | M01933 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody
View all Integrin alpha 2/CD49b Antibodies
SKU/Catalog Number
M01933
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody catalog # M01933. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01933)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GEB-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin alpha 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01933 is reactive to ITGA2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated molecular weight
129295 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01933 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:100
FC 1:100
Positive Control
IHC: Rat heart tissue, Human lung large cell cancer tissue, Mouse lung tissue, Rat liver tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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Western blot analysis of Integrin alpha 2 expression in A431 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:200 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ITGA2 (Source: Uniprot.org, NCBI)
Gene Name
ITGA2
Full Name
Integrin alpha-2
Weight
129295 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-2;CD49 antigen-like family member B;Collagen receptor;Platelet membrane glycoprotein Ia;GPIa;VLA-2 subunit alpha;CD49b;ITGA2;CD49B; ITGA2 BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2 integrin subunit alpha 2 integrin alpha-2|CD49 -like family member B|alpha 2 subunit of VLA-2 receptor|collagen receptor|human platelet allo system 5|integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)|platelet Br|platelet glycoprotein GPIa|platelet membrane glycoprotein Ia|very late activation protein 2 receptor, alpha-2 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGA2, check out the ITGA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody (M01933)
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No publications found for M01933
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Customer Q&As
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5 Customer Q&As for Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody
Question
Would anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 work on horse IP with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
Our lab technicians have not validated anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse leukemic t-cell in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-01
Question
We are currently using anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The anti-Integrin alpha 2 Rabbit Monoclonal antibody (M01933) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-12
Question
I am interested in to test anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 on human leukemic t-cell for research purposes, then I may be interested in using anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The products we sell, including anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-02
Question
I have a question about product M01933, anti-Integrin alpha 2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01933 anti-Integrin alpha 2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-02
Question
I was wanting to use your anti-Integrin alpha 2 Rabbit Monoclonal antibody for ICC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
J. Dhar
Verified customer
Asked: 2013-06-18
Answer
It shows on the product datasheet, M01933 anti-Integrin alpha 2 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-18
