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Product Info Summary
| SKU: | M00067-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC |
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Product info
Product Name
Anti-Insulin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00067-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Insulin Rabbit Monoclonal Antibody catalog # M00067-1. Tested in IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00067-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DEA-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Insulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00067-1 is reactive to INS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
11981 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00067-1 is guaranteed for IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:500-1:1000
ICC/IF 1:50-1:200
Positive Control
IHC: mouse pancreas tissue
ICC/IF: BxPC-3 cell
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded mouse pancreas, using Insulin Antibody.
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Immunofluorescent analysis of BxPC-3 cells, using Insulin Antibody .
Protein Target Info & Infographic
Gene/Protein Information For INS (Source: Uniprot.org, NCBI)
Gene Name
INS
Full Name
Insulin
Weight
11981 MW
Superfamily
insulin family
Alternative Names
Insulin;Insulin B chain;Insulin A chain;INS; INS IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4 insulin insulin|preproinsulin|proinsulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INS, check out the INS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insulin Rabbit Monoclonal Antibody (M00067-1)
Hello CJ!
M00067-1 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dong Yan,Pengcheng Fan,Wenlong Sun,Qianzhi Ding,Wei Zheng,Weidi Xiao,Bowei Zhang,Tao Zhang,Tao Zhang,Jiahui Shi,Xiaojuan Chen,Peiru Chen,Jie Zhang,Ying Hao,Xinguang Sun,Xu Pang,Yuesheng Dong,Ping Xu,Liyan Yu,Baiping Ma,Anemarrhena asphodeloides modulates
Species: Rat,A. asphodeloides
M00067-1 usage in article: APP:IF, SAMPLE:PANCREAS TISSUE, DILUTION:1:100
Huo K,Li X,Hu W,Song X,Zhang D,Zhang X,Chen X,Yuan J,Zuo J,Wang X.RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Front Endocrinol (Lausanne). 2020 Apr 9;11:194.doi:10.3389/fendo.2020.00194.PMID
Species: Rat
M00067-1 usage in article: APP:IHC, SAMPLE:PANCREATIC TISSUE, DILUTION:1:100
Gynura divaricata rich in 3, 5%u2212/4, 5-dicaffeoylquinic acid and chlorogenic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic %u2026
RIG-I inhibits pancreatic ? cell proliferation through competitive binding of activated Src
Interference effect of oral administration of mulberry branch bark powder on the incidence of type II diabetes in mice induced by streptozotocin
miR-487b mitigates chronic heart failure through inhibition of the IL-33/ST2 signaling pathway
Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of...
Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.
Hu N, Yuan L, Li Hj, Huang C, Mao Qm, Zhang Yy, Lin M, Sun Yq, Zhong Xy, Tang P, Lu X. Evid Based Complement Alternat Med. 2013;2013:180721. Doi: 10.1155/2013/180721. Epub 2013 May 29. Anti-Diabetic Activities Of Jiaotaiwan In Db/Db Mice By Augmen...
Ding H, Zhu T, Yin X, Liu J, Zhang L, Bernier M, Zhao R. Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):582-9. Doi: 10.1093/Abbs/Gmu034. Epub 2014 May 14. Pyrrolidine Dithiocarbamate Protects Pancreatic ??-Cells From Oxidative Damage Through ...
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-Insulin Rabbit Monoclonal Antibody
Question
Can we use basic antigen retrieval with M00067-1 antibody, as alternatives for citrate buffer?
Verified customer
Asked: 2019-10-24
Answer
For the Anti-Insulin Rabbit Monoclonal Antibody M00067-1, it is suggested to use Tris-EDTA buffer for the antigen retrieval buffer with the compositions; 10 mM Tris(hydroxymethyl)methyl aminomethane THAM, 1 mM EDTA, 0.05% Tween 20, pH 9.0.
Boster Scientific Support
Answered: 2019-10-29
Question
Would you please check the IHC image for this mAb: https://www.bosterbio.com/anti-insulin-rabbit-monoclonal-antibody-m00067-1-boster.html?options=cart I
Verified Customer
Verified customer
Asked: 2014-06-11
Answer
We have updated the image. https://www.bosterbio.com/anti-insulin-rabbit-monoclonal-antibody-m00067-1-boster.html?options=cart
Boster Scientific Support
Answered: 2014-06-11
