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Product Info Summary
| SKU: | M00067 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Cow, Human, Mouse, Pig, Rabbit |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Insulin / IRDN (beta-Cell & Insulinoma Marker) Monoclonal Antibody
SKU/Catalog Number
M00067
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Insulin / IRDN (beta-Cell & Insulinoma Marker) Monoclonal Antibody (Catalog # M00067). Tested in IHC applications. This antibody reacts with Human, Cow, Pig, Rabbit, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Insulin / IRDN (beta-Cell & Insulinoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00067)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: IRDN/805
Isotype
IgG1, kappa
Immunogen
Recombinant INS protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00067 is reactive to INS in Cow, Human, Mouse, Pig, Rabbit
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
11981 MW
Background of INS
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00067 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human pancreas stained with Anti-Insulin Monoclonal Antibody (IRDN/805).
Protein Target Info & Infographic
Gene/Protein Information For INS (Source: Uniprot.org, NCBI)
Gene Name
INS
Full Name
Insulin
Weight
11981 MW
Superfamily
insulin family
Alternative Names
Insulin;Insulin B chain;Insulin A chain;INS; INS IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4 insulin insulin|preproinsulin|proinsulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INS, check out the INS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insulin / IRDN (beta-Cell & Insulinoma Marker) Monoclonal Antibody (M00067)
Hello CJ!
M00067 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cotransplantation With Xenogenetic Neonatal Porcine Sertoli Cells Significantly Prolongs Islet Allograft Survival in Nonimmunosuppressive Rats
Attenuated Tregs increase susceptibility to type 1 diabetes in prenatal nicotine exposed female offspring mice
Liraglutide protects β-cell function by reversing histone modification of Pdx-1 proximal promoter in catch-up growth male rats
Reversible immortalization of rat pancreatic β cells with a novel immortalizing and tamoxifen-mediated self-recombination tricistronic vector
Gao T,Jiao Y,Liu Y,Li T,Wang Z,Wang D.Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.J Diabetes Res.2019 Oct 7;2019:3872182.doi:10.1155/2019/3872182.PMID:31687407;PMCID:PMC6800947.
Species: Rat
M00067 usage in article: APP:IHC, SAMPLE: PANCREASE, DILUTION:1:200
Heng Yang,Shuo Wang,Yingchun Ye,Min Xie,Yubin Li,Hong Ji,Jing Li,Ling Gao, GLP-1 preserves β cell function via improvement on islet insulin signaling in high fat diet feeding mice, Neuropeptides, 2020,102110,ISSN 0143-4179,https://doi.org/10.1016/j.npep.2
Species: Mouse
M00067 usage in article: APP:IF, SAMPLE:PANCREAS, DILUTION:NA
Gynura divaricata rich in 3, 5%u2212/4, 5-dicaffeoylquinic acid and chlorogenic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic %u2026
RIG-I inhibits pancreatic ? cell proliferation through competitive binding of activated Src
Interference effect of oral administration of mulberry branch bark powder on the incidence of type II diabetes in mice induced by streptozotocin
miR-487b mitigates chronic heart failure through inhibition of the IL-33/ST2 signaling pathway
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