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Product Info Summary
| SKU: | A01991 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Nephrin/NPHS1 Antibody Picoband®
SKU/Catalog Number
A01991
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Nephrin/NPHS1 Antibody Picoband® catalog # A01991. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nephrin/NPHS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01991)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Nephrin recombinant protein (Position: V1084-V1241). Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01991 is reactive to NPHS1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
185 kDa
Calculated molecular weight
134742 MW
Background of Nephrin
NPHS1, also called Nephrin, is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. It gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01991 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Positive Control
WB: rat kidney tissue, mouse kidney tissue
IHC: mouse kidney tissue, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Nephrin using anti-Nephrin antibody (A01991).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nephrin antigen affinity purified polyclonal antibody (Catalog # A01991) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nephrin at approximately 185 kDa. The expected band size for Nephrin is at 135 kDa.
Click image to see more details
Figure 2. IHC analysis of Nephrin using anti-Nephrin antibody (A01991).
Nephrin was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nephrin Antibody (A01991) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Nephrin using anti-Nephrin antibody (A01991).
Nephrin was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nephrin Antibody (A01991) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0003) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For NPHS1 (Source: Uniprot.org, NCBI)
Gene Name
NPHS1
Full Name
Nephrin
Weight
134742 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Nephrin;Renal glomerulus-specific cell adhesion receptor;NPHS1;NPHN; NPHS1 CNF, NPHN, nephrin NPHS1 adhesion molecule, nephrin nephrin|NPHS1, nephrin|nephrosis 1, congenital, Finnish type (nephrin)|renal glomerulus-specific cell adhesion receptor|truncated NPHS1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPHS1, check out the NPHS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPHS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nephrin/NPHS1 Antibody Picoband® (A01991)
Hello CJ!
A01991 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation
Qiu Y,Zhou J,Zhang H,Zhou H,Tang H,Lei C,Ye C,You C,Chen Y,Wang Y,Xiong J,Su H,Yao G,Zhang C.Rhodojaponin II attenuates kidney injury by regulating TGF-β1/Smad pathway in mice with adriamycin nephropathy.J Ethnopharmacol.2019 Oct 28;243:112078.doi:10.1016/j.jep.2019.112078.Epub 2019 Jul 10.PMID: 31301369.
Species: Mouse
A01991 usage in article: APP:WB, SAMPLE:RENAL TISSUE, DILUTION:1:200
Zhang X,Zhang L,Chen Z,Li S,Che B, Wang N,Chen J,Xu C,Wei C.Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.Int J Mol Med.2021 Mar;47(3):27.doi:10.3892/ijmm.2021.4860.Epub 2021 Feb 4.PMID:33537831.
Species: Rat
A01991 usage in article: APP:WB, SAMPLE:RENAL TISSUE AND PODOCYTES, DILUTION:1:400
Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
A01991 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:1:50
Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
Effect of simvastatin on the expression of nephrin, podocin, and vascular endothelial growth factor (VEGF) in podocytes of diabetic rat
Hao L, Pan Ms, Zheng Y, Wang Rf. Exp Ther Med. 2014 Jun;7(6):1465-1470. Epub 2014 Apr 7. Effect Of Cordyceps Sinensis And Tripterygium Wilfordii Polyglycosidium On Podocytes In Rats With Diabetic Nephropathy.
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Nephrin/NPHS1 Antibody Picoband®
Question
Has the anti-nephrin antibody Picoband (A01991) been tested for specificity? We are looking for very specific anti-nephrin antibodies, and are a bit worried about the change in the expected MW band.
Verified customer
Asked: 2022-12-14
Answer
Our lab performed WB and IHC-P tests and please see the result images on the product page. https://www.bosterbio.com/anti-nephrin-picoband-trade-antibody-a01991-boster.html We haven't run any other tests for specificity.
Boster Scientific Support
Answered: 2022-12-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for metanephric glomerulus using anti-Nephrin/NPHS1 antibody A01991. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-29
Question
Do you have a BSA free version of anti-Nephrin/NPHS1 antibody A01991 available?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Nephrin/NPHS1 antibody A01991 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-21
Question
We are currently using anti-Nephrin/NPHS1 antibody A01991 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
The anti-Nephrin/NPHS1 antibody (A01991) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-10
Question
I was wanting to use your anti-Nephrin/NPHS1 antibody for WB for mouse metanephric glomerulus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse metanephric glomerulus identification?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
As indicated on the product datasheet, A01991 anti-Nephrin/NPHS1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse metanephric glomerulus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
See below the WB image, lot number and protocol we used for metanephric glomerulus using anti-Nephrin/NPHS1 antibody A01991. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
We ordered your anti-Nephrin/NPHS1 antibody for WB on platelet in a previous experiment. I am using rat, and We are going to use the antibody for IHC next. I was wanting to use examining platelet as well as metanephric glomerulus in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
I viewed the website and datasheets of our anti-Nephrin/NPHS1 antibody and it appears that A01991 has been tested on rat in both WB and IHC. Thus A01991 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-26
Question
Is this A01991 anti-Nephrin/NPHS1 antibody reactive to the isotypes of NPHS1?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The immunogen of A01991 anti-Nephrin/NPHS1 antibody is E.coli-derived human Nephrin recombinant protein (Position: V1084-V1241). Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-Nephrin/NPHS1 antibody A01991 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-13
Question
I am looking for to test anti-Nephrin/NPHS1 antibody A01991 on mouse metanephric glomerulus for research purposes, then I may be interested in using anti-Nephrin/NPHS1 antibody A01991 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
The products we sell, including anti-Nephrin/NPHS1 antibody A01991, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-28
Question
Will anti-Nephrin/NPHS1 antibody A01991 work for WB with metanephric glomerulus?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
According to the expression profile of metanephric glomerulus, NPHS1 is highly expressed in metanephric glomerulus. So, it is likely that anti-Nephrin/NPHS1 antibody A01991 will work for WB with metanephric glomerulus.
Boster Scientific Support
Answered: 2018-05-18
Question
Would A01991 anti-Nephrin/NPHS1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
It shows on the product datasheet, A01991 anti-Nephrin/NPHS1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-08
Question
Can you help my question with product A01991, anti-Nephrin/NPHS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01991 anti-Nephrin/NPHS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-07
Question
Is a blocking peptide available for product anti-Nephrin/NPHS1 antibody (A01991)?
W. Zhang
Verified customer
Asked: 2017-12-22
Answer
We do provide the blocking peptide for product anti-Nephrin/NPHS1 antibody (A01991). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-22
Question
We have seen staining in mouse platelet. Are there any suggestions? Is anti-Nephrin/NPHS1 antibody supposed to stain platelet positively?
J. Mangal
Verified customer
Asked: 2016-11-14
Answer
From literature platelet does express NPHS1. From Uniprot.org, NPHS1 is expressed in metanephric glomerulus, platelet, among other tissues. Regarding which tissues have NPHS1 expression, here are a few articles citing expression in various tissues:
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2016-11-14
Question
My team were satisfied with the WB result of your anti-Nephrin/NPHS1 antibody. However we have observed positive staining in platelet cell membrane using this antibody. Is that expected? Could you tell me where is NPHS1 supposed to be expressed?
B. Yang
Verified customer
Asked: 2015-07-30
Answer
From literature, platelet does express NPHS1. Generally NPHS1 expresses in cell membrane. Regarding which tissues have NPHS1 expression, here are a few articles citing expression in various tissues:
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2015-07-30
Question
We want using your anti-Nephrin/NPHS1 antibody for excretion studies. Has this antibody been tested with western blotting on 293t whole cell lysates? We would like to see some validation images before ordering.
K. Roberts
Verified customer
Asked: 2013-03-18
Answer
I appreciate your inquiry. This A01991 anti-Nephrin/NPHS1 antibody is tested on rat kidney tissue, mouse kidney tissue, 293t whole cell lysates. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-03-18
