Click image to see more details
Product Info Summary
| SKU: | A00102-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL6 Antibody Picoband®
SKU/Catalog Number
A00102-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL6 Antibody Picoband® catalog # A00102-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00102-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6, which shares 87.5% amino acid (aa) sequence identity with mouse IL6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00102-2 is reactive to Il6 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
30748 MW
Background of IL-6
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, the IL6 gene is mapped to chromosome 7p15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00102-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat spleen tissue, mouse spleen tissue, mouse Ana-1 whole cell, mouse RAW2647 whole cell, mouse EL-4 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (A00102-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse Ana-1 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates,
Lane 5: mouse EL-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # A00102-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Il6 (Source: Uniprot.org, NCBI)
Gene Name
Il6
Full Name
Interleukin-6
Weight
30748 MW
Superfamily
IL-6 superfamily
Alternative Names
Interleukin-6; IL-6; Il6; Il-6 IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il6, check out the Il6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL6 Antibody Picoband® (A00102-2)
Hello CJ!
A00102-2 has been cited in 27 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Driving GABAergic neurons optogenetically improves learning, reduces amyloid load and enhances autophagy in a mouse model of Alzheimer’s disease
Tan J,Luo J,Meng C,Jiang N,Cao J,Zhao J. Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway. Int Immunopharmacol.2020 Dec 11;90:107268.doi:10.1016/j. intimp.2020.107268.Epub ahead of print.PMID:333
Species: Rat
A00102-2 usage in article: APP:WB, SAMPLE:CEREBRAL CORTEX, DILUTION:NA
Potential Ameliorative Effects of Qing Ye Dan Against Cadmium Induced Prostatic Deficits via Regulating Nrf-2/HO-1 and TGF-%u03B21/Smad Pathways
Transfection with CXCR4 potentiates homing of mesenchymal stem cells in vitro and therapy of diabetic retinopathy in vivo
The expression and significance of IL-6, IFN-%u03B3, SM22%u03B1, and MMP-2 in rat model of aortic dissection
Distribution change of mast cells in human nasal polyps
The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway
Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm
Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats
Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-IL6 Antibody Picoband®
Question
I would like using your anti-IL6 antibody for response to mechanical stimulus studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate your inquiry. This A00102-2 anti-IL6 antibody is tested on rat heart tissue, tissue lysate, mouse heart tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-20
Question
I have attached the WB image, lot number and protocol we used for fibroblast using anti-IL6 antibody A00102-2. Please let me know if you require anything else.
J. Evans
Verified customer
Asked: 2020-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
Do you have a BSA free version of anti-IL6 antibody A00102-2 available?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL6 antibody A00102-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-10
Question
We bought anti-IL6 antibody for WB on left coronary artery in a previous experiment. I am using rat, and I plan to use the antibody for ELISA next. My question regards examining left coronary artery as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
I have checked the website and datasheets of our anti-IL6 antibody and it appears that A00102-2 has been validated on rat in both WB and ELISA. Thus A00102-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-IL6 antibody A00102-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
Will A00102-2 anti-IL6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can see on the product datasheet, A00102-2 anti-IL6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-10
Question
We have seen staining in rat lung. What should we do? Is anti-IL6 antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
Based on literature lung does express IL6. Based on Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-15
Question
I see that the anti-IL6 antibody A00102-2 works with WB, what is the protocol used to produce the result images on the product page?
D. Zhang
Verified customer
Asked: 2019-02-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-26
Question
My question regarding product A00102-2, anti-IL6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00102-2 anti-IL6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-26
Question
Is this A00102-2 anti-IL6 antibody reactive to the isotypes of IL6?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
The immunogen of A00102-2 anti-IL6 antibody is A synthetic peptide corresponding to a sequence of rat IL6 (KTIQLILKALEEFLKVTMRSTRQT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-12
Question
Our lab were well pleased with the WB result of your anti-IL6 antibody. However we have seen positive staining in fibroblast secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
From literature, fibroblast does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-25
Question
I am interested in to test anti-IL6 antibody A00102-2 on rat fibroblast for research purposes, then I may be interested in using anti-IL6 antibody A00102-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
The products we sell, including anti-IL6 antibody A00102-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-12
Question
I was wanting to use your anti-IL6 antibody for WB for rat fibroblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fibroblast identification?
J. Wu
Verified customer
Asked: 2015-01-15
Answer
It shows on the product datasheet, A00102-2 anti-IL6 antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-15
Question
Does anti-IL6 antibody A00102-2 work for WB with fibroblast?
J. Moore
Verified customer
Asked: 2014-01-14
Answer
According to the expression profile of fibroblast, IL6 is highly expressed in fibroblast. So, it is likely that anti-IL6 antibody A00102-2 will work for WB with fibroblast.
Boster Scientific Support
Answered: 2014-01-14
Question
We are currently using anti-IL6 antibody A00102-2 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
K. Gonzalez
Verified customer
Asked: 2013-08-27
Answer
The anti-IL6 antibody (A00102-2) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-27
Question
Is a blocking peptide available for product anti-IL6 antibody (A00102-2)?
Z. Krishna
Verified customer
Asked: 2013-05-14
Answer
We do provide the blocking peptide for product anti-IL6 antibody (A00102-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-14
