Product Info Summary
SKU: | P11872 |
---|---|
Size: | 100ug |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody
SKU/Catalog Number
P11872
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody (Catalog # P11872). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P11872)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P11872 is reactive to Sephs2 in Mouse
Applications
P11872 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
47305 MW
Background of Sephs2
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase (SelD) catalyzes the conversion of selenium to selenophosphate which is required by a number of bacterial, archaeal and eukaryotic organisms for synthesis of selenocysteine-tRNA, the precursor of selenocysteine in selenoenzymes. A second selenophosphate synthetase (SPS2) was identified in mammals. SPS2 is itself a selenoprotein in mammals.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot using Protein A purified anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Protein Target Info & Infographic
Gene/Protein Information For Sephs2 (Source: Uniprot.org, NCBI)
Gene Name
Sephs2
Full Name
Selenide, water dikinase 2
Weight
47305 MW
Superfamily
selenophosphate synthase 1 family
Alternative Names
EC 2.7.9.3; Selenophosphate synthase 2; selenophosphate synthetase 2; SPS2water dikinase 2 Sephs2|S, Sps2, Ysg, Ysg3|selenophosphate synthetase 2|selenide, water dikinase 2|selenium donor protein 2|selenophosphate synthase 2|the SelD gene product
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sephs2, check out the Sephs2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sephs2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody (P11872)
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No publications found for P11872
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Customer Q&As
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7 Customer Q&As for Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody
Question
See below the WB image, lot number and protocol we used for muscle skin using anti-Selenophosphate Synthetase 2 antibody P11872. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-27
Question
I was wanting to use your anti-Selenophosphate Synthetase 2 antibody for WB for mouse muscle skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse muscle skin identification?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
You can see on the product datasheet, P11872 anti-Selenophosphate Synthetase 2 antibody has been validated for IP, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse muscle skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-11
Question
Is there a BSA free version of anti-Selenophosphate Synthetase 2 antibody P11872 available?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Selenophosphate Synthetase 2 antibody P11872 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-23
Question
I have a question about product P11872, anti-Selenophosphate Synthetase 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P11872 anti-Selenophosphate Synthetase 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-Selenophosphate Synthetase 2 antibody P11872 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on pig tissues as well?
G. Jha
Verified customer
Asked: 2016-05-20
Answer
The anti-Selenophosphate Synthetase 2 antibody (P11872) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-20
Question
Will P11872 anti-Selenophosphate Synthetase 2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Walker
Verified customer
Asked: 2016-03-31
Answer
As indicated on the product datasheet, P11872 anti-Selenophosphate Synthetase 2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-31
Question
We need to test anti-Selenophosphate Synthetase 2 antibody P11872 on mouse muscle skin for research purposes, then I may be interested in using anti-Selenophosphate Synthetase 2 antibody P11872 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Krishna
Verified customer
Asked: 2013-01-21
Answer
The products we sell, including anti-Selenophosphate Synthetase 2 antibody P11872, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-01-21