Mouse IL7R/CD127 ELISA Kit PicoKine®

IL-7R alpha/CD127 ELISA kit for Mouse

Mouse IL7R/CD127 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il7r in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK0714
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IL7R/CD127 ELISA Kit PicoKine®

View all IL-7R alpha/CD127 ELISA kits

SKU/Catalog Number

EK0714

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL7R/CD127 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il7r in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL7R/CD127 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0714)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: E21-D239

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK0714 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0714 is reactive to Il7r in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0714 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-7R alpha/CD127

The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

Kit Components

Catalog Number Description Quantity
EK0714-CAP Anti-Mouse Il7r Pre-coated 96-well strip microplate 1
EK0714-ST Mouse Il7r Standard 2 vials, 20 ng/tube
EK0714-DA Mouse Il7r Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0600.1350.2140.3380.5680.8931.4611.912

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-7R alpha/CD127, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 31.2 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL7R/CD127 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6627096461293970
Standard deviation2.7718.0974.223.5322.2682.45
CV (%)4.2%6.7%7.7%5.8%7.6%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-7R alpha/CD127 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 166776568694.746.8%
Sample 227025728728827512.854.6%
Sample 396491290081389754.266%
*number of samples for each test n=16.

Gene/Protein Information For Il7r (Source: Uniprot.Org, NCBI)

Gene Name

Il7r

Full Name

Interleukin-7 receptor subunit alpha

Weight

51605 MW

Superfamily

type I cytokine receptor family

Alternative Names

Interleukin-7 receptor subunit alpha;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;CD127;Il7r; IL7R CD127, CDW127, IL-7R-alphaA, ILRA, IL7R interleukin 7 receptor interleukin-7 receptor subunit alpha|CD127 |IL-7 receptor subunit alpha|IL-7R subunit alpha|interleukin 7 receptor alpha chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il7r, check out the Il7r Infographic

Il7r infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il7r: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Mouse IL7R/CD127 ELISA Kit PicoKine®

Question

Q: how to analyze ELISA data? I measured CD127 level in serum.

C. Banerjee

Verified customer

Asked: 2020-09-25

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-09-25

Question

For the kit EK0714 a potential customer asks for your in-house testing data with mouse serum samples. Would you have this information available to share?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

COA is sent.

Boster Scientific Support

Answered: 2020-04-16

Question

Have you tested EK0714 kit with mouse serum and can it be returned if it is not working with mouse serum upon purchase?

Verified customer

Asked: 2019-09-25

Answer

The Mouse IL7R/CD127 ELISA Kit PicoKine EK0714 has been validated for mouse cell culture supernates and serum samples. If the kit is not performing as expected for the validated species and applications listed on the product page, then our lab technicians will troubleshoot and if they find that there is a quality issue with the kit, then we will provide a replacement of another lot or a refund for the kit.

Boster Scientific Support

Answered: 2019-09-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-06-19

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD127 using the EK0714 Mouse CD127 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-06-12

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-06-12

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Maharaj

Verified customer

Asked: 2018-06-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-10

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

T. Williams

Verified customer

Asked: 2018-05-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-05-24

Question

Q: if the enzyme conjugated CD127 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD127 antigen?

Verified Customer

Verified customer

Asked: 2016-05-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-05-23

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