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Product Info Summary
| SKU: | A00118-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-IKK beta IKBKB Antibody
SKU/Catalog Number
A00118-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IKK beta IKBKB Antibody (Catalog # A00118-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
IKK beta antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IKK beta IKBKB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00118-1)
Host
Rabbit
Contents
IKK beta Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid. The immunogen is located within amino acids 650 - 700 of IKK beta.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This polyclonal antibody has no cross response to IKKa or IKKy.
Reactive Species
A00118-1 is reactive to IKBKB in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
86564 MW
Background of IKK beta
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for NF-κB activation. IKKβ phosphorylates both IκB-alpha and IκB-beta. IKKβ is expressed in variety of human tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00118-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μg/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
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Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 μg/mL.
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Immunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IKBKB (Source: Uniprot.org, NCBI)
Gene Name
IKBKB
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit beta
Weight
86564 MW
Superfamily
protein kinase superfamily
Alternative Names
IKK2, IKKB, IMD15, NFKBIKB, IKK-beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, I-kappa-B-kinase beta IKBKB IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB inhibitor of nuclear factor kappa B kinase subunit beta inhibitor of nuclear factor kappa-B kinase subunit beta|I-kappa-B kinase 2|I-kappa-B-kinase beta|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta|nuclear factor NF-kappa-B inhibitor kinase beta|serine/threonine protein kinase IKBKB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKBKB, check out the IKBKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKBKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKK beta IKBKB Antibody (A00118-1)
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