Key=store_id
Key=entity_id, value=3310
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Key=type_id, value=simple
Key=sku, value=EK1421
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Key=created_at, value=2015-10-20 18:34:38
Key=updated_at, value=2024-09-26 00:46:50
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human CD47 ELISA Kit PicoKine®
Key=cj_search_index
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Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
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Key=featured, value=No
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Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=elisa-kits
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Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1365
Key=yoast_content_score, value=60
Key=price, value=750.0000
Key=weight, value=1.0000
Key=meta_title, value=Human CD47 ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Human CD47 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD47 in cell culture supernatants and serum.. Sensitivity: 10pg/ml.
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Key=small_image, value=/e/k/ek1421_2.png
Key=thumbnail, value=/e/k/ek1421_2.png
Key=url_key, value=human-cd47-picokine-trade-elisa-kit-ek1421-boster
Key=url_path, value=human-cd47-picokine-trade-elisa-kit-ek1421-boster
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Key=image_label, value=Human CD47 PicoKine ELISA Kit standard curve
Key=small_image_label, value=Human CD47 PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Human CD47 PicoKine ELISA Kit standard curve
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Key=size, value=96 wells/kit, with removable strips.
Key=protein_name, value=Leukocyte surface antigen CD47
Key=gene_full_name, value=Leukocyte surface antigen CD47
Key=uniprot_id, value=Q08722
Key=assay_range, value=125 pg/ml - 8,000 pg/ml
Key=sensitivity, value=<10 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=monoclonal antibody from mouse|polyclonal antibody from goat
Key=assay_procedure_step_1, value=8,000 pg/ml, 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=predicted_reactivity
Key=source_company, value=Boster EK1421
Key=molecular_weight, value=35214 MW
Key=related_product_skus, value=A00360-1,M00360,PA2223,PROTQ08722,PROTQ08722-1
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Cancer, Cell Type Markers, Immunology, Tumor Immunology
Key=swatch_image, value=/e/k/ek1421_2.png
Key=rating_value, value=99.2
Key=review_count, value=24
Key=phospho_site
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Key=cj_related_products, value=Anemia, Hemolytic:PB10033|A00914-3|PB9487|FC00910-FITC|FC00910-PE|EK1948
Key=description, value=Human CD47 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD47 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Human CD47 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD47 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Human CD47 ELISA Kit PicoKine, Bosterbio High Sensitivity
Key=background, value=CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.
Key=gene_name, value=CD47
Key=synonyms, value=Leukocyte surface antigen CD47;Antigenic surface determinant protein OA3;Integrin-associated protein;IAP;Protein MER6;CD47;CD47;MER6;
Key=sample_type, value=cell culture supernatants and serum.
Key=kit_components, value=10 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	193	888	3988	208	813	3951
Standard deviation	7.72	54.16	183.44	11.02	60.97	256.81
CV (%)	4%	6.1%	4.6%	5.3%	7.5%	6.5%

Key=application_details, value=Dilution ratio of 1:1, concentration in serum and plasma is around 40 pg/ml.
Key=subcellular_localization, value= Cell membrane ; Multi-pass membrane protein .
Key=sequence_similarities, value=Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Key=tissue_specificity, value= Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Key=protein_function, value= Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. .
Key=sample_data, value=

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.038	0.081	0.161	0.187	0.333	0.586	0.902	1.764

Key=competitor_equivalent_skus, value=sc 12731|sc 7059|sc 25773|sc 21786|sc 7057|sc 59079|sc 12730|sc 53050|sc 70716
Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: Q19-P139
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	193	162	183	189	181	11.94	6.5%
Sample 2	888	960	1024	1007	969	52.69	5.4%
Sample 3	3988	4066	3941	3907	3975	59.64	1.5%

*number of samples for each test n=16.
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