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Product Info Summary
| SKU: | M07469 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal Antibody
SKU/Catalog Number
M07469
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal Antibody (Catalog # M07469). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07469)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: Cocktail
Isotype
IgG1, kappa + IgG2b, kappa
Immunogen
Heavy chain of human IgM
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M07469 is reactive to IGHM in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
49307 MW
Background of IGHM
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07469 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-IgM Mouse Monoclonal Antibody (Cocktail)
Protein Target Info & Infographic
Gene/Protein Information For IGHM (Source: Uniprot.org, NCBI)
Gene Name
IGHM
Full Name
Immunoglobulin heavy constant mu
Weight
49307 MW
Alternative Names
Ig mu chain C region;IGHM; IGHM AGM1, MU, VH immunoglobulin heavy constant mu constant region of heavy chain of IgM|immunoglobulin mu chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHM, check out the IGHM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal Antibody (M07469)
Hello CJ!
M07469 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Weihong Zeng,Guangfeng Liu,Huan Ma,Dan Zhao,Yunru Yang,Muziying Liu,Ahmed Mohammed,Changcheng Zhao,Yun Yang,Jiajia Xie,Chengchao Ding,Xiaoling Ma,Jianping Weng,Yong Gao,Hongliang He,Tengchuan Jin,Biochemical characterization of SARS-CoV-2 nucleocapsid protein,Biochemical and Biophysical Research Communications,Volume 527,Issue 3,2020,Pages 618-623,ISSN 0006-291X,https://doi.org/10.1016/j.bbrc.2020.04.136.
Species: Human
M07469 usage in article: APP:WB, SAMPLE:SERUM, DILUTION:NA
Rat adipose-derived stem cells express low level of ?-Gal and are dependent on CD59 for protection from human xenoantibody and complement-mediated lysis
An outbreak caused by GII.17 norovirus with a wide spectrum of HBGA-associated susceptibility
Ansari AR, Li NY, Sun ZJ, Huang HB, Zhao X, Cui L, Hu YF, Zhong JM, Karrow NA, Liu HZ. Oncotarget. 2017 Aug 5;8(65):108375-108391. doi: 10.18632/oncotarget.19964. eCollection 2017 Dec 12. Lipopolysaccharide induces acute bursal atrophy in broiler ...
Guan W, Wang Y, Hou L, Chen L, Li X, Yue W, Ma Y. Poult Sci. 2010 Feb;89(2):312-7. Doi: 10.3382/Ps.2009-00413. Derivation And Characteristics Of Pluripotent Embryonic Germ Cells In Duck.
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal Antibody
Question
I was wondering if there is a recommendation for the HIER conditions in the IHC-P protocol for M07469?
Verified customer
Asked: 2022-02-23
Answer
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Boster Scientific Support
Answered: 2022-02-28
Question
Is this M07469 anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody reactive to the isotypes of IGHM?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The immunogen of M07469 anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody is Recombinant heavy chain of human IgM (IM260 & IM373); Heavy chain of human IgM (ICO-30). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-25
Question
I see that the anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody M07469 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-14
Question
Is there a BSA free version of anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody M07469 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody M07469 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
My question regarding product M07469, anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M07469 anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
I was wanting to use your anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody for IHC for human lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph node identification?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
As indicated on the product datasheet, M07469 anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-17
Question
My lab would like to test anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody M07469 on human lymph node for research purposes, then I may be interested in using anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody M07469 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Mitchell
Verified customer
Asked: 2017-10-19
Answer
The products we sell, including anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody M07469, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-19
Question
Does M07469 anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Bhatt
Verified customer
Asked: 2015-07-22
Answer
As indicated on the product datasheet, M07469 anti-IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-22
