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Product Info Summary
| SKU: | M07469-3 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC |
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Product info
Product Name
Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121
SKU/Catalog Number
M07469-3
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (Catalog # M07469-3). Tested in ELISA, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07469-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM121
Isotype
Rabbit IgG
Immunogen
Human IgM
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the heavy chain of human IgM. No cross-reactivity with human IgG, IgA, IgD, or IgE.
Reactive Species
M07469-3 is reactive to IGHM in Human
Reconstitution
Calculated molecular weight
49440 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07469-3 is guaranteed for ELISA, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Validation Images & Assay Conditions
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Figure 1. ELISA result showing specificity
Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Figure 2. ELISA result showing specificity
Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Figure 3. ELISA result showing specificity
ELISA of human immunoglobulins shows RM121 only reacted to human IgM. No cross reactivity with IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Figure 4. ELISA result showing specificity
A titer ELISA using RM121. The plate was coated with different amounts of human IgM. A serial dilution of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For IGHM (Source: Uniprot.org, NCBI)
Gene Name
IGHM
Full Name
Immunoglobulin heavy constant mu
Weight
49440 MW
Alternative Names
Immunoglobulin heavy constant mu ;Ig mu chain C region ;Ig mu chain C region BOT ;Ig mu chain C region GAL ;Ig mu chain C region OU ;IGHM ; IGHM AGM1, MU, VH immunoglobulin heavy constant mu constant region of heavy chain of IgM|immunoglobulin mu chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHM, check out the IGHM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (M07469-3)
Hello CJ!
M07469-3 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Rat adipose-derived stem cells express low level of ?-Gal and are dependent on CD59 for protection from human xenoantibody and complement-mediated lysis
An outbreak caused by GII.17 norovirus with a wide spectrum of HBGA-associated susceptibility
Ansari AR, Li NY, Sun ZJ, Huang HB, Zhao X, Cui L, Hu YF, Zhong JM, Karrow NA, Liu HZ. Oncotarget. 2017 Aug 5;8(65):108375-108391. doi: 10.18632/oncotarget.19964. eCollection 2017 Dec 12. Lipopolysaccharide induces acute bursal atrophy in broiler ...
Guan W, Wang Y, Hou L, Chen L, Li X, Yue W, Ma Y. Poult Sci. 2010 Feb;89(2):312-7. Doi: 10.3382/Ps.2009-00413. Derivation And Characteristics Of Pluripotent Embryonic Germ Cells In Duck.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121
Question
Does M07469-3 cross react with murine IgM?
Verified customer
Asked: 2022-03-06
Answer
No, Our Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (M07469-3) does not cross react with murine IgM.
Boster Scientific Support
Answered: 2022-03-06
Question
What is the suggested dilution ratio for ICC and IHC of M07469-3?
Verified customer
Asked: 2019-08-12
Answer
For the Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone RM121 (M07469-3), the suggested dilution ratio for Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-Human IgM Rabbit Monoclonal antibody, Clone#RM121 M07469-3 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
The anti-Human IgM Rabbit Monoclonal antibody, Clone#RM121 (M07469-3) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-14
