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Product Info Summary
| SKU: | A00070-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-IGF-1R (Ab-1161) Antibody
View all IGF-I R/IGF1R Antibodies
SKU/Catalog Number
A00070-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-IGF-1R (Ab-1161) Antibody (Catalog # A00070-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGF-1R (Ab-1161) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00070-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.1159~1163 (D-I-Y-E-T) derived from Human IGF-1R .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00070-1 is reactive to IGF1R in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
30619 MW
Background of IGF-I R/IGF1R
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.
Li S, et al. (1994) J Biol Chem; 269(51).
Hernandez-Sanchez C, et al. (1995) J Biol Chem.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00070-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 200 95 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IGF1R using anti-IGF1R antibody (A00070-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF1R antigen affinity purified polyclonal antibody (Catalog # A00070-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IGF1R.
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Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R(Ab-1161) Antibody #A00070-1.
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Figure 2. IHC analysis of IGF1R using anti-IGF1R antibody (A00070-1).
IGF1R was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IGF1R Antibody (A00070-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IGF1R (Source: Uniprot.org, NCBI)
Gene Name
IGF1R
Full Name
Insulin-like growth factor 1 receptor
Weight
30619 MW
Superfamily
protein kinase superfamily
Alternative Names
Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5; IGF1R CD221, IGFIR, IGFR, JTK13 insulin like growth factor 1 receptor insulin-like growth factor 1 receptor|IGF-I receptor|soluble IGF1R variant 1|soluble IGF1R variant 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGF1R, check out the IGF1R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGF1R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGF-1R (Ab-1161) Antibody (A00070-1)
Hello CJ!
A00070-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of IGF-1R, VEGF-C and D2-40 and their correlation with lymph node metastasis in endometrial adenocarcinoma
Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway
Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome
IGF-1/IGF-1R/hsa-let-7c axis regulates the committed differentiation of stem cells from apical papilla
Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression
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Customer Q&As
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16 Customer Q&As for Anti-IGF-1R (Ab-1161) Antibody
Question
Is this A00070-1 anti-IGF-1R (Ab-1161) antibody reactive to the isotypes of IGF1R?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen of A00070-1 anti-IGF-1R (Ab-1161) antibody is Peptide sequence around aa.1159~1163 (D-I-Y-E-T) derived from Human IGF-1R . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-30
Question
My team were happy with the WB result of your anti-IGF-1R (Ab-1161) antibody. However we have been able to see positive staining in fetal brain cell membrane using this antibody. Is that expected? Could you tell me where is IGF1R supposed to be expressed?
D. Huang
Verified customer
Asked: 2020-04-24
Answer
From what I have seen in literature, fetal brain does express IGF1R. Generally IGF1R expresses in cell membrane. Regarding which tissues have IGF1R expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Fetal brain, Pubmed ID: 16572171
Melanocyte, Pubmed ID: 8247543, 1846292
Placenta, Pubmed ID: 2877871, 8257688
Boster Scientific Support
Answered: 2020-04-24
Question
We are currently using anti-IGF-1R (Ab-1161) antibody A00070-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The anti-IGF-1R (Ab-1161) antibody (A00070-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-16
Question
Is a blocking peptide available for product anti-IGF-1R (Ab-1161) antibody (A00070-1)?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
We do provide the blocking peptide for product anti-IGF-1R (Ab-1161) antibody (A00070-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-13
Question
We have seen staining in rat melanocyte. Any tips? Is anti-IGF-1R (Ab-1161) antibody supposed to stain melanocyte positively?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
Based on literature melanocyte does express IGF1R. Based on Uniprot.org, IGF1R is expressed in caput epididymis, placenta, fetal brain, melanocyte, cervix carcinoma, among other tissues. Regarding which tissues have IGF1R expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Fetal brain, Pubmed ID: 16572171
Melanocyte, Pubmed ID: 8247543, 1846292
Placenta, Pubmed ID: 2877871, 8257688
Boster Scientific Support
Answered: 2019-09-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-IGF-1R (Ab-1161) antibody A00070-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
I was wanting to use your anti-IGF-1R (Ab-1161) antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
It shows on the product datasheet, A00070-1 anti-IGF-1R (Ab-1161) antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-15
Question
you antibody using your anti-IGF-1R (Ab-1161) antibody for irs-related events triggered by igf1r studies. Has this antibody been tested with western blotting on mcf cells? We would like to see some validation images before ordering.
J. Baker
Verified customer
Asked: 2019-05-06
Answer
Thanks for your inquiry. This A00070-1 anti-IGF-1R (Ab-1161) antibody is tested on mcf cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-06
Question
I have a question about product A00070-1, anti-IGF-1R (Ab-1161) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00070-1 anti-IGF-1R (Ab-1161) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-11
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-IGF-1R (Ab-1161) antibody A00070-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-18
Question
I see that the anti-IGF-1R (Ab-1161) antibody A00070-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-11
Question
We have tried in the past anti-IGF-1R (Ab-1161) antibody for WB on fetal brain in a previous experiment. I am using human, and We intend to use the antibody for IF next. We are interested in examining fetal brain as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
I have checked the website and datasheets of our anti-IGF-1R (Ab-1161) antibody and I see that A00070-1 has been validated on human in both WB and IF. Thus A00070-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-17
Question
I was wanting to use to test anti-IGF-1R (Ab-1161) antibody A00070-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-IGF-1R (Ab-1161) antibody A00070-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Wu
Verified customer
Asked: 2016-11-03
Answer
The products we sell, including anti-IGF-1R (Ab-1161) antibody A00070-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-03
Question
Would anti-IGF-1R (Ab-1161) antibody A00070-1 work for WB with cervix carcinoma?
S. Parker
Verified customer
Asked: 2016-08-05
Answer
According to the expression profile of cervix carcinoma, IGF1R is highly expressed in cervix carcinoma. So, it is likely that anti-IGF-1R (Ab-1161) antibody A00070-1 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2016-08-05
Question
Do you have a BSA free version of anti-IGF-1R (Ab-1161) antibody A00070-1 available?
C. Evans
Verified customer
Asked: 2014-01-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IGF-1R (Ab-1161) antibody A00070-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-01-03
Question
Would A00070-1 anti-IGF-1R (Ab-1161) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Banerjee
Verified customer
Asked: 2013-08-16
Answer
You can see on the product datasheet, A00070-1 anti-IGF-1R (Ab-1161) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-16
