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Product Info Summary
| SKU: | M00134 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Huntingtin Monoclonal Antibody
View all Huntingtin Antibodies
SKU/Catalog Number
M00134
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Huntingtin Monoclonal Antibody catalog # M00134. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Huntingtin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00134)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADGC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Huntingtin May play a role in microtubule-mediated transport or vesicle function.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00134 is reactive to HTT in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
82785 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00134 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:100
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Huntingtin expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For HTT (Source: Uniprot.org, NCBI)
Gene Name
HTT
Full Name
Huntingtin
Weight
82785 MW
Superfamily
huntingtin family
Alternative Names
Transcriptional repressor CTCF;11-zinc finger protein;CCCTC-binding factor;CTCFL paralog;CTCF; HTT HD, IT15, LOMARS huntingtin huntingtin|huntington disease protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTT, check out the HTT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Huntingtin Monoclonal Antibody (M00134)
Hello CJ!
No publications found for M00134
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Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Huntingtin Monoclonal Antibody
Question
Would anti-Huntingtin Monoclonal antibody M00134 work on canine IF with retina?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Our lab technicians have not tested anti-Huntingtin Monoclonal antibody M00134 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Huntingtin Monoclonal antibody M00134 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine retina in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
We have observed staining in rat cerebellum. Any tips? Is anti-Huntingtin Monoclonal antibody supposed to stain cerebellum positively?
C. Kulkarni
Verified customer
Asked: 2019-10-15
Answer
According to literature cerebellum does express HTT. According to Uniprot.org, HTT is expressed in cerebellum, retina, brain, brain, caudate nucleus, frontal cortex, muscle retina, platelet, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have HTT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10332029, 11013077
Brain, Caudate nucleus, Frontal cortex, Muscle, and Retina, Pubmed ID: 7903579
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Retina, Pubmed ID: 8458085
Boster Scientific Support
Answered: 2019-10-15
Question
I was wanting to use your anti-Huntingtin Monoclonal antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
You can see on the product datasheet, M00134 anti-Huntingtin Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-19
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-Huntingtin Monoclonal antibody M00134. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-06
Question
My colleagues were happy with the WB result of your anti-Huntingtin Monoclonal antibody. However we have seen positive staining in muscle retina huntingtin using this antibody. Is that expected? Could you tell me where is HTT supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
From what I have seen in literature, muscle retina does express HTT. Generally HTT expresses in huntingtin: cytoplasm, huntingtin, myristoylated n-terminal. Regarding which tissues have HTT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10332029, 11013077
Brain, Caudate nucleus, Frontal cortex, Muscle, and Retina, Pubmed ID: 7903579
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Retina, Pubmed ID: 8458085
Boster Scientific Support
Answered: 2019-08-21
Question
Is this M00134 anti-Huntingtin Monoclonal antibody reactive to the isotypes of HTT?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The immunogen of M00134 anti-Huntingtin Monoclonal antibody is A synthesized peptide derived from human Huntingtin May play a role in microtubule-mediated transport or vesicle function. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-12
Question
Will anti-Huntingtin Monoclonal antibody M00134 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
According to the expression profile of leukemic t-cell, HTT is highly expressed in leukemic t-cell. So, it is likely that anti-Huntingtin Monoclonal antibody M00134 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-02-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Huntingtin Monoclonal antibody M00134. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-31
Question
We are currently using anti-Huntingtin Monoclonal antibody M00134 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
The anti-Huntingtin Monoclonal antibody (M00134) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-29
Question
Is there a BSA free version of anti-Huntingtin Monoclonal antibody M00134 available?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Huntingtin Monoclonal antibody M00134 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-26
Question
We purchased anti-Huntingtin Monoclonal antibody for IF on brain in the past. I am using rat, and We intend to use the antibody for WB next. I was wanting to use examining brain as well as retina in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
E. Mangal
Verified customer
Asked: 2018-02-09
Answer
I have checked the website and datasheets of our anti-Huntingtin Monoclonal antibody and it seems that M00134 has been validated on rat in both IF and WB. Thus M00134 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-02-09
Question
Would M00134 anti-Huntingtin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
It shows on the product datasheet, M00134 anti-Huntingtin Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-30
Question
I was wanting to use to test anti-Huntingtin Monoclonal antibody M00134 on rat leukemic t-cell for research purposes, then I may be interested in using anti-Huntingtin Monoclonal antibody M00134 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Parker
Verified customer
Asked: 2017-11-30
Answer
The products we sell, including anti-Huntingtin Monoclonal antibody M00134, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-30
Question
I have a question about product M00134, anti-Huntingtin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00134 anti-Huntingtin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-14
Question
Is a blocking peptide available for product anti-Huntingtin Monoclonal antibody (M00134)?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
We do provide the blocking peptide for product anti-Huntingtin Monoclonal antibody (M00134). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-08
Question
I see that the anti-Huntingtin Monoclonal antibody M00134 works with WB, what is the protocol used to produce the result images on the product page?
C. Evans
Verified customer
Asked: 2014-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-07-03
