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Product Info Summary
| SKU: | EK1189 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse MSP/MST1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1189
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MSP/MST1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mst1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MSP/MST1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1189)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q19-E716
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1189 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1189 is reactive to Mst1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1189 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MSP/MST1
Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1189-CAP | Anti-Mouse Mst1 Pre-coated 96-well strip microplate | 1 |
| EK1189-ST | Mouse Mst1 Standard | 2 vials, 10 ng/tube |
| EK1189-DA | Mouse Mst1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.047 | 0.071 | 0.105 | 0.177 | 0.313 | 0.569 | 1.059 | 1.881 |
Data Example Images
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Mouse MSP/MST1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MSP/MST1, Dilution ratio of 1:500, concentration in serum and plasma is around 1000 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MSP/MST1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 257 | 1513 | 5860 | 276 | 1456 | 5996 |
| Standard deviation | 11.56 | 62.03 | 322.3 | 12.97 | 61.15 | 425.71 |
| CV (%) | 4.5% | 4.1% | 5.5% | 4.7% | 4.2% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MSP/MST1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 257 | 283 | 261 | 264 | 266 | 9.98 | 3.7% |
| Sample 2 | 1513 | 1499 | 1287 | 1336 | 1408 | 98.9 | 7% |
| Sample 3 | 5860 | 5664 | 5841 | 5939 | 5826 | 100.49 | 1.7% |
Protein Target Info & Infographic
Gene/Protein Information For Mst1 (Source: Uniprot.Org, NCBI)
Gene Name
Mst1
Full Name
Hepatocyte growth factor-like protein
Weight
80619 MW
Superfamily
peptidase S1 family
Alternative Names
Hepatocyte growth factor-like protein;Macrophage stimulatory protein;MSP;Hepatocyte growth factor-like protein alpha chain;Hepatocyte growth factor-like protein beta chain;Mst1;Hgfl; MST1 D3F15S2, DNF15S2, HGFL, MSP, NF15S2 macrophage stimulating 1 hepatocyte growth factor-like protein|hepatocyte growth factor-like protein homolog|macrophage stimulating 1 (hepatocyte growth factor-like)|macrophage-stimulating protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Mst1, check out the Mst1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mst1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse MSP/MST1 ELISA Kit PicoKine® (EK1189)
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Customer Q&As
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6 Customer Q&As for Mouse MSP/MST1 ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for MSP ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MSP for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-13
Question
Q: how do I analyze ELISA data? I have obtained MSP level in serum.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-12-26
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
T. Chen
Verified customer
Asked: 2019-07-07
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-07
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification MSP using the EK1189 Mouse MSP Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-04-30
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
A. Liu
Verified customer
Asked: 2016-07-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-07-02
Question
Q: can I use citrate plasma as samples in Mouse MSP Picokine® ELISA Kit (Catalog # EK1189)?
V. Mitchell
Verified customer
Asked: 2014-09-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of MSP causing degradation of its protein structure. MSP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MSP ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-09-29
