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Product Info Summary
| SKU: | A00291-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-ICOS Antibody
SKU/Catalog Number
A00291-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ICOS Antibody catalog # A00291-3. Tested in ELISA, Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICOS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00291-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ICOS recombinant protein (Position: E21-K140).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00291-3 is reactive to ICOS in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
22.625kDa
Background of ICOS
Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00291-3 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
IHC: human tonsil tissue
FCM: Raji cell
Validation Images & Assay Conditions
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Figure 1. IHC analysis of ICOS using anti-ICOS antibody (A00291-3).
ICOS was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICOS Antibody (A00291-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Flow Cytometry analysis of Raji cells using anti-ICOS antibody (A00291-3).
Overlay histogram showing Raji cells stained with A00291-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICOS Antibody (A00291-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ICOS (Source: Uniprot.org, NCBI)
Gene Name
ICOS
Full Name
Inducible T-cell costimulator
Weight
22.625kDa
Alternative Names
Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; ICOS; AILIM ICOS AILIM, CD278, CVID1 inducible T cell costimulator inducible T-cell costimulator|activation-inducible lymphocyte immunomediatory molecule|inducible T-cell co-stimulator|inducible costimulator
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICOS, check out the ICOS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICOS Antibody (A00291-3)
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Customer Q&As
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16 Customer Q&As for Anti-ICOS Antibody
Question
I was wanting to use your anti-ICOS antibody for ICC for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
You can see on the product datasheet, A00291-3 anti-ICOS antibody has been tested for ELISA, Flow Cytometry, IHC, ICC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-03
Question
Do you have a BSA free version of anti-ICOS antibody A00291-3 available?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ICOS antibody A00291-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-20
Question
My lab would like using your anti-ICOS antibody for t cell costimulation studies. Has this antibody been tested with western blotting on raji cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your inquiry. This A00291-3 anti-ICOS antibody is validated on human tonsil tissue, raji cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC, ICC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-31
Question
I see that the anti-ICOS antibody A00291-3 works with ICC, what is the protocol used to produce the result images on the product page?
V. Rodriguez
Verified customer
Asked: 2019-10-18
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-18
Question
We have seen staining in human blood. Any tips? Is anti-ICOS antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
Based on literature blood does express ICOS. Based on Uniprot.org, ICOS is expressed in leukocyte, blood, thymus, among other tissues. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774
Boster Scientific Support
Answered: 2019-10-14
Question
We are currently using anti-ICOS antibody A00291-3 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-ICOS antibody (A00291-3) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
Our team were well pleased with the WB result of your anti-ICOS antibody. However we have seen positive staining in leukocyte isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ICOS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
From what I have seen in literature, leukocyte does express ICOS. Generally ICOS expresses in isoform 1: cell membrane. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774
Boster Scientific Support
Answered: 2019-09-16
Question
Would A00291-3 anti-ICOS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
It shows on the product datasheet, A00291-3 anti-ICOS antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-ICOS antibody A00291-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-12
Question
Can you help my question with product A00291-3, anti-ICOS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Wu
Verified customer
Asked: 2019-05-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00291-3 anti-ICOS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-21
Question
I have attached the WB image, lot number and protocol we used for leukocyte using anti-ICOS antibody A00291-3. Please let me know if you require anything else.
A. Jones
Verified customer
Asked: 2018-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-18
Question
Would anti-ICOS antibody A00291-3 work for ICC with leukocyte?
M. Evans
Verified customer
Asked: 2017-02-28
Answer
According to the expression profile of leukocyte, ICOS is highly expressed in leukocyte. So, it is likely that anti-ICOS antibody A00291-3 will work for ICC with leukocyte.
Boster Scientific Support
Answered: 2017-02-28
Question
I would like to test anti-ICOS antibody A00291-3 on human leukocyte for research purposes, then I may be interested in using anti-ICOS antibody A00291-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Patel
Verified customer
Asked: 2016-07-18
Answer
The products we sell, including anti-ICOS antibody A00291-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-07-18
Question
We bought anti-ICOS antibody for Flow Cytometry on thymus in a previous project. I am using human, and We intend to use the antibody for IHC next. I would like examining thymus as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
F. Wu
Verified customer
Asked: 2015-05-05
Answer
I took a look at the website and datasheets of our anti-ICOS antibody and it seems that A00291-3 has been validated on human in both Flow Cytometry and IHC. Thus A00291-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-05-05
Question
Is this A00291-3 anti-ICOS antibody reactive to the isotypes of ICOS?
P. Carter
Verified customer
Asked: 2014-09-12
Answer
The immunogen of A00291-3 anti-ICOS antibody is E. coli-derived human ICOS recombinant protein (Position: E21-K140). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-09-12
Question
Is a blocking peptide available for product anti-ICOS antibody (A00291-3)?
J. Yang
Verified customer
Asked: 2014-07-14
Answer
We do provide the blocking peptide for product anti-ICOS antibody (A00291-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-07-14
