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Product Info Summary
| SKU: | A03125-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TNFRSF18 Antibody Picoband®
View all GITR/TNFRSF18 Antibodies
SKU/Catalog Number
A03125-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNFRSF18 Antibody Picoband® catalog # A03125-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNFRSF18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03125-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TNFRSF18 recombinant protein (Position: L178-V241). Human TNFRSF18 shares 76.2% amino acid (aa) sequence identity with mouse TNFRSF18.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03125-1 is reactive to TNFRSF18 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
26000 MW
Background of GITR/TNFRSF18
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03125-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TNFRSF18 using anti-TNFRSF18 antibody (A03125-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysate,
Lane 2: mouse kidney tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF18 antigen affinity purified polyclonal antibody (Catalog # A03125-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF18 at approximately 28KD. The expected band size for TNFRSF18 is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF18 (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF18
Full Name
Tumor necrosis factor receptor superfamily member 18
Weight
26000 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 18;Activation-inducible TNFR family receptor;Glucocorticoid-induced TNFR-related protein;CD357;TNFRSF18;AITR, GITR;UNQ319/PRO364; TNFRSF18 AITR, CD357, ENERGEN, GITR, GITR-D TNF receptor superfamily member 18 tumor necrosis factor receptor superfamily member 18|TNF receptor superfamily activation-inducible protein|activation-inducible TNFR family receptor|glucocorticoid-induced TNFR-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF18, check out the TNFRSF18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNFRSF18 Antibody Picoband® (A03125-1)
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Customer Q&As
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6 Customer Q&As for Anti-TNFRSF18 Antibody Picoband®
Question
Is there a BSA free version of anti-TNFRSF18 antibody A03125-1 available?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TNFRSF18 antibody A03125-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-04
Question
I see that the anti-TNFRSF18 antibody A03125-1 works with WB, what is the protocol used to produce the result images on the product page?
B. Krishna
Verified customer
Asked: 2020-02-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-07
Question
Does A03125-1 anti-TNFRSF18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
You can see on the product datasheet, A03125-1 anti-TNFRSF18 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-12
Question
Can you help my question with product A03125-1, anti-TNFRSF18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Kulkarni
Verified customer
Asked: 2018-01-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03125-1 anti-TNFRSF18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-03
Question
Does anti-TNFRSF18 antibody A03125-1 work for WB with t-cell?
N. Dhar
Verified customer
Asked: 2015-09-28
Answer
According to the expression profile of t-cell, TNFRSF18 is highly expressed in t-cell. So, it is likely that anti-TNFRSF18 antibody A03125-1 will work for WB with t-cell.
Boster Scientific Support
Answered: 2015-09-28
Question
My lab would like to test anti-TNFRSF18 antibody A03125-1 on rat t-cell for research purposes, then I may be interested in using anti-TNFRSF18 antibody A03125-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Moore
Verified customer
Asked: 2014-01-14
Answer
The products we sell, including anti-TNFRSF18 antibody A03125-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-01-14
