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Product Info Summary
| SKU: | M05613-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Beta-tubulin TUBB4A Monoclonal Antibody
View all Tubulin Beta 4 Antibodies
SKU/Catalog Number
M05613-3
Size
100ul
Description
Boster Bio Anti-Beta-tubulin TUBB4A Monoclonal Antibody catalog # M05613-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Beta-tubulin TUBB4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05613-3)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Pig brain tubulin preparation
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05613-3 is reactive to TUBB4A in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
49586 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05613-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000-1:10,000. IF: 1:5,000.
Validation Images & Assay Conditions
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Western blot analysis of whole tissue or cell lysates using mouse mAb to -tubulin, M05613-3, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50kDa corresponds to the -tubulin proteins.
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Immunofluorescent analysis of HeLa cells stained with mouse mAb to β-tubulin, M05613-3, dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. M05613-3 antibody produces strong staining of microtubules in the cytoplasm, while the other antibody labels the nuclei of HeLa cells. Mouse select each image for larger view.
Protein Target Info & Infographic
Gene/Protein Information For TUBB4A (Source: Uniprot.org, NCBI)
Gene Name
TUBB4A
Full Name
Tubulin beta-4A chain
Weight
49586 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta-4A chain;Tubulin 5 beta;Tubulin beta-4 chain;TUBB4A;TUBB4, TUBB5; TUBB4A DYT4, TUBB4, beta-5 tubulin beta 4A class IVa tubulin beta-4A chain|dystonia 4, torsion (autosomal dominant)|tubulin beta-4 chain|tubulin, beta 4|tubulin, beta, 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB4A, check out the TUBB4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beta-tubulin TUBB4A Monoclonal Antibody (M05613-3)
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Customer Q&As
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7 Customer Q&As for Anti-Beta-tubulin TUBB4A Monoclonal Antibody
Question
Does anti- Beta-tubulin Monoclonal antibody M05613-3 work for IHC with right hemisphere of cerebellum?
K. Moore
Verified customer
Asked: 2019-12-11
Answer
According to the expression profile of right hemisphere of cerebellum, TUBB4A is highly expressed in right hemisphere of cerebellum. So, it is likely that anti- Beta-tubulin Monoclonal antibody M05613-3 will work for IHC with right hemisphere of cerebellum.
Boster Scientific Support
Answered: 2019-12-11
Question
Do you have a BSA free version of anti- Beta-tubulin Monoclonal antibody M05613-3 available?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti- Beta-tubulin Monoclonal antibody M05613-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-08
Question
I have attached the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti- Beta-tubulin Monoclonal antibody M05613-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-24
Question
Is this M05613-3 anti- Beta-tubulin Monoclonal antibody reactive to the isotypes of TUBB4A?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
The immunogen of M05613-3 anti- Beta-tubulin Monoclonal antibody is Pig brain tubulin preparation. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-22
Question
Does anti- Beta-tubulin Monoclonal antibody M05613-3 work on zebrafish WB with brain uterus?
J. Jha
Verified customer
Asked: 2018-07-18
Answer
Our lab technicians have not validated anti- Beta-tubulin Monoclonal antibody M05613-3 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti- Beta-tubulin Monoclonal antibody M05613-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-18
Question
We are currently using anti- Beta-tubulin Monoclonal antibody M05613-3 for monkey tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
S. Rodriguez
Verified customer
Asked: 2015-09-04
Answer
The anti- Beta-tubulin Monoclonal antibody (M05613-3) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-04
Question
My question regarding product M05613-3, anti- Beta-tubulin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Huang
Verified customer
Asked: 2013-09-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05613-3 anti- Beta-tubulin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-09-26
