Click image to see more details
Product Info Summary
| SKU: | FC00220-APC |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD86 Monoclonal Antibody APC Conjugated, Flow Validated
SKU/Catalog Number
FC00220-APC
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD86 Monoclonal Antibody APC Conjugated, Flow Validated (Catalog# FC00220-APC). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD86 Monoclonal Antibody APC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00220-APC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: BU63
Isotype
IgG1,k
Immunogen
B-lymphoblastoid cell line ARH 77
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00220-APC is reactive to CD86 in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
37682 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00220-APC is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Monocytes gated PBMCs stained with APC conjugated anti-human CD86 (clone BU63) (red histogram). Monocytes gated PBMCs stained with APC conjugated mouse IgG1 isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)
Gene Name
CD86
Full Name
T-lymphocyte activation antigen CD86
Weight
37682 MW
Alternative Names
T-lymphocyte activation antigen CD86;Activation B7-2 antigen;B70;BU63;CTLA-4 counter-receptor B7.2;FUN-1;CD86;CD86;CD28LG2; CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation CD86|B-lymphocyte activation B7-2|BU63|CD86 (CD28 ligand 2, B7-2 )|CTLA-4 counter-receptor B7.2|FUN-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD86, check out the CD86 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD86 Monoclonal Antibody APC Conjugated, Flow Validated (FC00220-APC)
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No publications found for FC00220-APC
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Customer Q&As
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11 Customer Q&As for Anti-Human CD86 Monoclonal Antibody APC Conjugated, Flow Validated
Question
Is there a BSA free version of anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC available?
A. Miller
Verified customer
Asked: 2020-02-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-18
Question
I was wanting to use your anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated for Flow Cytometry for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
As indicated on the product datasheet, FC00220-APC anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
Is a blocking peptide available for product anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated (FC00220-APC)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We do provide the blocking peptide for product anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated (FC00220-APC). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-13
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-21
Question
Does anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC work for Flow Cytometry with leukocyte?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
According to the expression profile of leukocyte, CD86 is highly expressed in leukocyte. So, it is likely that anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC will work for Flow Cytometry with leukocyte.
Boster Scientific Support
Answered: 2018-03-13
Question
Would FC00220-APC anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Baker
Verified customer
Asked: 2017-12-15
Answer
As indicated on the product datasheet, FC00220-APC anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-15
Question
I see that the anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
S. Krishna
Verified customer
Asked: 2017-04-18
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-04-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC. Let me know if you need anything else.
B. Li
Verified customer
Asked: 2016-10-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-31
Question
Is this FC00220-APC anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated reactive to the isotypes of CD86?
C. Patel
Verified customer
Asked: 2014-12-12
Answer
The immunogen of FC00220-APC anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated is B-lymphoblastoid cell line ARH 77. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-12-12
Question
I would like to test anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC on human leukocyte for research purposes, then I may be interested in using anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Banerjee
Verified customer
Asked: 2014-11-20
Answer
The products we sell, including anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated FC00220-APC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-20
Question
Can you help my question with product FC00220-APC, anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Thomas
Verified customer
Asked: 2013-02-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00220-APC anti-Human CD86 Monoclonal antibody APC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-27
