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Product Info Summary
| SKU: | FC00214-APC |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD25 IL2RA Monoclonal Antibody APC Conjugated, Flow Validated
View all CD25/IL-2R alpha Antibodies
SKU/Catalog Number
FC00214-APC
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD25 IL2RA Monoclonal Antibody APC Conjugated, Flow Validated (Catalog# FC00214-APC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD25 IL2RA Monoclonal Antibody APC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00214-APC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 7G7B6
Isotype
IgG2a,k
Immunogen
Influenza virus stimulated human peripheral blood mononuclear cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00214-APC is reactive to IL2RA in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
30819 MW
Background of CD25/IL-2R alpha
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00214-APC is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PHA stimulated (3days) fresh PBMCs stained with APC conjugated mouse antihuman CD25 (clone 7G7B6) along with FITC conjugated anti-human CD4 (right panel). Lymphocytes gated PHA stimulated PBMCs stained with isotype control mouse IgG2a APC and antihuman CD4 FITC (left panel).
Protein Target Info & Infographic
Gene/Protein Information For IL2RA (Source: Uniprot.org, NCBI)
Gene Name
IL2RA
Full Name
Interleukin-2 receptor subunit alpha
Weight
30819 MW
Alternative Names
Interleukin-2 receptor subunit alpha;IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2-RA;TAC antigen;p55;CD25;IL2RA; IL2RA CD25, IDDM10, IL2R, IMD41, TCGFR, p55 interleukin 2 receptor subunit alpha interleukin-2 receptor subunit alpha|IL-2 receptor subunit alpha|IL-2R subunit alpha|TAC |interleukin 2 receptor, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL2RA, check out the IL2RA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL2RA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD25 IL2RA Monoclonal Antibody APC Conjugated, Flow Validated (FC00214-APC)
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