Click image to see more details
Product Info Summary
| SKU: | FC03780 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-human CD20 MS4A1 Monoclonal Antibody Unconjugated, Flow Validated
SKU/Catalog Number
FC03780
Size
100ug, 500ug
Form
Liquid
Description
Boster Bio Anti-human CD20 MS4A1 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC03780). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD20 MS4A1 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC03780)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 2H7
Isotype
IgG2b,k
Immunogen
Human tonsillar B cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC03780 is reactive to MS4A1 in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Calculated molecular weight
33077 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC03780 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated human PBMCs stained with anti-human CD20 (clone 2H7) followed by Goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Blue histogram is for the PBMCs stained with only Goat anti-mouse IgG (H+L) Alexa Fluor 488.
Protein Target Info & Infographic
Gene/Protein Information For MS4A1 (Source: Uniprot.org, NCBI)
Gene Name
MS4A1
Full Name
B-lymphocyte antigen CD20
Weight
33077 MW
Superfamily
MS4A family
Alternative Names
B-lymphocyte antigen CD20;B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;CD20;MS4A1;CD20; MS4A1 B1, Bp35, CD20, CVID5, FMC7, LEU-16, MS4A2, S7 membrane spanning 4-domains A1 B-lymphocyte CD20|B-lymphocyte cell-surface B1|CD20 |CD20 receptor|leukocyte surface Leu-16|membrane-spanning 4-domains, subfamily A, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MS4A1, check out the MS4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MS4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD20 MS4A1 Monoclonal Antibody Unconjugated, Flow Validated (FC03780)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-human CD20 MS4A1 Monoclonal Antibody Unconjugated, Flow Validated?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-human CD20 MS4A1 Monoclonal Antibody Unconjugated, Flow Validated
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Anti-human CD20 MS4A1 Monoclonal Antibody Unconjugated, Flow Validated
Question
I have attached the WB image, lot number and protocol we used for t-cell using anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-17
Question
Does FC03780 anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
As indicated on the product datasheet, FC03780 anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-11
Question
I see that the anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-20
Question
Does anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780 work for Flow Cytometry with t-cell?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
According to the expression profile of t-cell, MS4A1 is highly expressed in t-cell. So, it is likely that anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780 will work for Flow Cytometry with t-cell.
Boster Scientific Support
Answered: 2019-06-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-04
Question
Can you help my question with product FC03780, anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC03780 anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-08
Question
Do you have a BSA free version of anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780 available?
A. Lewis
Verified customer
Asked: 2016-03-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-03-09
Question
Is this FC03780 anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated reactive to the isotypes of MS4A1?
A. Evans
Verified customer
Asked: 2015-08-07
Answer
The immunogen of FC03780 anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated is Human tonsillar B cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-08-07
Question
My question regards to test anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780 on human t-cell for research purposes, then I may be interested in using anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Wu
Verified customer
Asked: 2015-04-08
Answer
The products we sell, including anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated FC03780, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-08
Question
I was wanting to use your anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated for Flow Cytometry for human t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human t-cell identification?
M. Moore
Verified customer
Asked: 2014-11-17
Answer
You can see on the product datasheet, FC03780 anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-17
Question
Is a blocking peptide available for product anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated (FC03780)?
J. Singh
Verified customer
Asked: 2013-12-26
Answer
We do provide the blocking peptide for product anti-human CD20 Monoclonal antibody Unconjugated, Flow Validated (FC03780). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-26
