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Product Info Summary
| SKU: | A00307-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RAG1 Antibody Picoband®
SKU/Catalog Number
A00307-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAG1 Antibody Picoband® catalog # A00307-1. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAG1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00307-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAG1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00307-1 is reactive to RAG1 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
63525 MW
Background of RAG1
Recombination activating gene 1 also known as RAG-1 is a protein that in humans is encoded by the RAG1 gene. The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00307-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse
Positive Control
WB: human Jurkat whole cell, human Jurkat whole cell, mouse thymus tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RAG1 using anti-RAG1 antibody (A00307-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAG1 antigen affinity purified polyclonal antibody (Catalog # A00307-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAG1 at approximately 120KD. The expected band size for RAG1 is at 120KD.
Protein Target Info & Infographic
Gene/Protein Information For RAG1 (Source: Uniprot.org, NCBI)
Gene Name
RAG1
Full Name
V(D)J recombination-activating protein 1
Weight
63525 MW
Superfamily
RAG1 family
Alternative Names
Interferon alpha/beta receptor 1;IFN-R-1;IFN-alpha/beta receptor 1;Cytokine receptor class-II member 1;Cytokine receptor family 2 member 1;CRF2-1;Type I interferon receptor 1;IFNAR1;IFNAR; RAG1 RAG-1, RNF74 recombination activating 1 V(D)J recombination-activating protein 1|RING finger protein 74|recombination activating gene 1|recombination activating protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAG1, check out the RAG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAG1 Antibody Picoband® (A00307-1)
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