Product Info Summary
SKU: | A03780-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD20/MS4A1 Antibody Picoband™
SKU/Catalog Number
A03780-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD20/MS4A1 Antibody Picoband™ catalog # A03780-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD20/MS4A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03780-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD20/MS4A1 recombinant protein (Position: M1-P291).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03780-3 is reactive to MS4A1 in Mouse
Applications
A03780-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
33 kDa
Calculated molecular weight
64133 MW
Background of CD20
CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What’s more, this protein may be involved in the regulation of B-cell activation and proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Flow Cytometry, 1-3μg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
![a03780 3 cd20 primary antibodies wb testing 1 a03780 3 cd20 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03780-3-cd20-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (A03780-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD20/MS4A1 antigen affinity purified polyclonal antibody (Catalog # A03780-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD20/MS4A1 at approximately 33KD. The expected band size for CD20/MS4A1 is at 33KD.
![a03780 3 cd20 primary antibodies ihc testing 2 a03780 3 cd20 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a03780-3-cd20-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (A03780-3).
CD20/MS4A1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD20/MS4A1 Antibody (A03780-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03780 3 cd20 primary antibodies ihc testing 3 a03780 3 cd20 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a03780-3-cd20-primary-antibodies-ihc-testing-3.jpg)
Click image to see more details
Figure 3. IHC analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (A03780-3).
CD20/MS4A1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD20/MS4A1 Antibody (A03780-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03780 3 cd20 primary antibodies fcm testing 4 a03780 3 cd20 primary antibodies fcm testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a03780-3-cd20-primary-antibodies-fcm-testing-4.png)
Click image to see more details
Figure 4. Flow Cytometry analysis of HEPA1-6 cells using anti-CD20/MS4A1 antibody (A03780-3).
Overlay histogram showing HEPA1-6 cells stained with A03780-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD20/MS4A1 Antibody (A03780-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MS4A1 (Source: Uniprot.org, NCBI)
Gene Name
MS4A1
Full Name
B-lymphocyte antigen CD20
Weight
64133 MW
Superfamily
MS4A family
Alternative Names
B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7 MS4A1 B1, Bp35, CD20, CVID5, FMC7, LEU-16, MS4A2, S7 membrane spanning 4-domains A1 B-lymphocyte antigen CD20|B-lymphocyte cell-surface antigen B1|CD20 antigen|CD20 receptor|leukocyte surface antigen Leu-16|membrane-spanning 4-domains, subfamily A, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MS4A1, check out the MS4A1 Infographic
![MS4A1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MS4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD20/MS4A1 Antibody Picoband™ (A03780-3)
Hello CJ!
A03780-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation:
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD20/MS4A1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD20/MS4A1 Antibody Picoband™
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question