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Product Info Summary
| SKU: | M03780-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD20 MS4A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03780-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD20 MS4A1 Rabbit Monoclonal Antibody catalog # M03780-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD20 MS4A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03780-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AIA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD20
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03780-1 is reactive to MS4A1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
33077 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03780-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Positive Control
IHC: human tonsil tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using CD20 Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of CD20 expression in Raji cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MS4A1 (Source: Uniprot.org, NCBI)
Gene Name
MS4A1
Full Name
B-lymphocyte antigen CD20
Weight
33077 MW
Superfamily
MS4A family
Alternative Names
B-lymphocyte antigen CD20;B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;CD20;MS4A1;CD20; MS4A1 B1, Bp35, CD20, CVID5, FMC7, LEU-16, MS4A2, S7 membrane spanning 4-domains A1 B-lymphocyte CD20|B-lymphocyte cell-surface B1|CD20 |CD20 receptor|leukocyte surface Leu-16|membrane-spanning 4-domains, subfamily A, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MS4A1, check out the MS4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MS4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD20 MS4A1 Rabbit Monoclonal Antibody (M03780-1)
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CD20 MS4A1 Rabbit Monoclonal Antibody
Question
Will M03780-1 anti-CD20 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
As indicated on the product datasheet, M03780-1 anti-CD20 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-05
Question
We have been able to see staining in human t-cell. Are there any suggestions? Is anti-CD20 Rabbit Monoclonal antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
From what I have seen in literature t-cell does express MS4A1. From what I have seen in Uniprot.org, MS4A1 is expressed in blood, lymph, t-cell, b-cell, among other tissues. Regarding which tissues have MS4A1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 22615937
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2020-01-29
Question
My question regards to test anti-CD20 Rabbit Monoclonal antibody M03780-1 on human lymph for research purposes, then I may be interested in using anti-CD20 Rabbit Monoclonal antibody M03780-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The products we sell, including anti-CD20 Rabbit Monoclonal antibody M03780-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-18
Question
I see that the anti-CD20 Rabbit Monoclonal antibody M03780-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-17
Question
We were content with the WB result of your anti-CD20 Rabbit Monoclonal antibody. However we have observed positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is MS4A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
From literature, blood does express MS4A1. Generally MS4A1 expresses in cell membrane. Regarding which tissues have MS4A1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 22615937
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-10-10
Question
Our lab used your anti-CD20 Rabbit Monoclonal antibody for IHC on t-cell a few years ago. I am using human, and I plan to use the antibody for WB next. you antibody examining t-cell as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
I looked at the website and datasheets of our anti-CD20 Rabbit Monoclonal antibody and it appears that M03780-1 has been tested on human in both IHC and WB. Thus M03780-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-02
Question
My question regarding product M03780-1, anti-CD20 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03780-1 anti-CD20 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-CD20 Rabbit Monoclonal antibody M03780-1. Let me know if you need anything else.
N. Kulkarni
Verified customer
Asked: 2018-01-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-23
Question
Is there a BSA free version of anti-CD20 Rabbit Monoclonal antibody M03780-1 available?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD20 Rabbit Monoclonal antibody M03780-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-26
Question
Is a blocking peptide available for product anti-CD20 Rabbit Monoclonal antibody (M03780-1)?
Verified Customer
Verified customer
Asked: 2017-08-18
Answer
We do provide the blocking peptide for product anti-CD20 Rabbit Monoclonal antibody (M03780-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-18
Question
Would anti-CD20 Rabbit Monoclonal antibody M03780-1 work for IHC with lymph?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
According to the expression profile of lymph, MS4A1 is highly expressed in lymph. So, it is likely that anti-CD20 Rabbit Monoclonal antibody M03780-1 will work for IHC with lymph.
Boster Scientific Support
Answered: 2017-06-28
Question
Will anti-CD20 Rabbit Monoclonal antibody M03780-1 work on canine IHC with t-cell?
A. Lewis
Verified customer
Asked: 2017-02-08
Answer
Our lab technicians have not tested anti-CD20 Rabbit Monoclonal antibody M03780-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD20 Rabbit Monoclonal antibody M03780-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-08
Question
Please see the WB image, lot number and protocol we used for lymph using anti-CD20 Rabbit Monoclonal antibody M03780-1. Please let me know if you require anything else.
B. Evans
Verified customer
Asked: 2016-11-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-08
Question
Is this M03780-1 anti-CD20 Rabbit Monoclonal antibody reactive to the isotypes of MS4A1?
M. Collins
Verified customer
Asked: 2015-09-21
Answer
The immunogen of M03780-1 anti-CD20 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD20. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-21
Question
We are currently using anti-CD20 Rabbit Monoclonal antibody M03780-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
V. Jones
Verified customer
Asked: 2015-04-20
Answer
The anti-CD20 Rabbit Monoclonal antibody (M03780-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-20
Question
I was wanting to use your anti-CD20 Rabbit Monoclonal antibody for IHC for human lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymph identification?
B. Patel
Verified customer
Asked: 2014-07-07
Answer
It shows on the product datasheet, M03780-1 anti-CD20 Rabbit Monoclonal antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-07
Question
I am interested in using your anti-CD20 Rabbit Monoclonal antibody for b cell receptor signaling pathway studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
D. Evans
Verified customer
Asked: 2013-03-29
Answer
Thanks for your inquiry. This M03780-1 anti-CD20 Rabbit Monoclonal antibody is tested on raji cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-03-29
