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Product Info Summary
| SKU: | A02428-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-FCGR1A Antibody Picoband®
View all Fc gamma RI/CD64 Antibodies
SKU/Catalog Number
A02428-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FCGR1A Antibody Picoband® catalog # A02428-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FCGR1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02428-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FCGR1A recombinant protein (Position: Q16-H292).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02428-1 is reactive to FCGR1A in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
38779 MW
Background of Fc gamma RI/CD64
High affinity immunoglobulin gamma Fc receptor I is a protein that in humans is encoded by the FCGR1A gene. It is mapped to 1q21.2. This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02428-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human SiHa whole cell, human A431 whole cell, human HCCT tissue, human Hela whole cell, rat PC-12 whole cell
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FCGR1A using anti-FCGR1A antibody (A02428-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HCCT tissue lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR1A at approximately 70 kDa. The expected band size for FCGR1A is at 43 kDa.
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Figure 2. Flow Cytometry analysis of U937 cells using anti-FCGR1A antibody (A02428-1).
Overlay histogram showing U937 cells stained with A02428-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FCGR1A Antibody (A02428-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For FCGR1A (Source: Uniprot.org, NCBI)
Gene Name
FCGR1A
Full Name
High affinity immunoglobulin gamma Fc receptor I
Weight
38779 MW
Superfamily
immunoglobulin superfamily
Alternative Names
High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD64; FCGR1A; FCG1; FCGR1; IGFR1 FCGR1A CD64, CD64A, FCRI, IGFR1 Fc fragment of IgG receptor Ia high affinity immunoglobulin gamma Fc receptor I|Fc fragment of IgG, high affinity Ia, receptor (CD64)|Fc fragment of IgG, high affinity Ia, receptor for (CD64)|Fc gamma receptor Ia|Fc-gamma RI|Fc-gamma receptor I A1|IgG Fc receptor I|fc-gamma RIA|fcgammaRIa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCGR1A, check out the FCGR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCGR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FCGR1A Antibody Picoband® (A02428-1)
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No publications found for A02428-1
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Customer Q&As
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4 Customer Q&As for Anti-FCGR1A Antibody Picoband®
Question
Is a blocking peptide available for product anti-FCGR1A antibody (A02428-1)?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do provide the blocking peptide for product anti-FCGR1A antibody (A02428-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-24
Question
We are currently using anti-FCGR1A antibody A02428-1 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-FCGR1A antibody (A02428-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
Question
We need to test anti-FCGR1A antibody A02428-1 on human substantia nigra for research purposes, then I may be interested in using anti-FCGR1A antibody A02428-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Jha
Verified customer
Asked: 2018-05-28
Answer
The products we sell, including anti-FCGR1A antibody A02428-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-FCGR1A antibody A02428-1. Let me know if you need anything else.
W. Wu
Verified customer
Asked: 2017-11-15
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-15
