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Product Info Summary
| SKU: | M01022 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-CD35 / CR1 (Follicular Dendritic Cell Marker) Monoclonal Antibody
SKU/Catalog Number
M01022
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD35 / CR1 (Follicular Dendritic Cell Marker) Monoclonal Antibody (Catalog # M01022). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Baboon, Cynomolgus monkey, Rhesus monkey.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD35 / CR1 (Follicular Dendritic Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01022)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM554
Isotype
IgG1, kappa
Immunogen
Intact human monocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01022 is reactive to CR1 in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
223663 MW
Background of CD35
Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01022 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD35 Mouse Monoclonal Antibody (SPM554).
Protein Target Info & Infographic
Gene/Protein Information For CR1 (Source: Uniprot.org, NCBI)
Gene Name
CR1
Full Name
Complement receptor type 1
Weight
223663 MW
Superfamily
receptors of complement activation (RCA) family
Alternative Names
Complement receptor type 1;C3b/C4b receptor;CD35;CR1;C3BR; CR1 C3BR, C4BR, CD35, KN complement C3b/C4b receptor 1 (Knops blood group) complement receptor type 1|C3-binding protein|C3b/C4b receptor|CD35 |Knops blood group |complement component (3b/4b) receptor 1 (Knops blood group)|complement receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CR1, check out the CR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD35 / CR1 (Follicular Dendritic Cell Marker) Monoclonal Antibody (M01022)
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