Click image to see more details
Product Info Summary
| SKU: | FC04535-FITC |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated
SKU/Catalog Number
FC04535-FITC
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC04535-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC04535-FITC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: FUT4/815
Isotype
IgM,k
Immunogen
Purified human neutrophils
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC04535-FITC is reactive to FUT4 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
59084 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC04535-FITC is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 5μl is enough to stain 1 million PBMCs or 100μl whole blood sample
Validation Images & Assay Conditions
Click image to see more details
Anti-human CD15 FITC, Flow Validated
Protein Target Info & Infographic
Gene/Protein Information For FUT4 (Source: Uniprot.org, NCBI)
Gene Name
FUT4
Full Name
Alpha-(1,3)-fucosyltransferase 4
Weight
59084 MW
Superfamily
glycosyltransferase 10 family
Alternative Names
Alpha- (1,3)-fucosyltransferase 4;2.4.1.-;ELAM-1 ligand fucosyltransferase;Fucosyltransferase 4;Fucosyltransferase IV;Fuc-TIV;FucT-IV;Galactoside 3-L-fucosyltransferase;FUT4;ELFT, FCT3A; FUT4 CD15, ELFT, FCT3A, FUC-TIV, FUTIV, LeX, SSEA-1 fucosyltransferase 4 alpha-(1,3)-fucosyltransferase 4|ELAM ligand fucosyltransferase|ELAM-1 ligand fucosyltransferase|Lewis X|alpha (1,3) fucosyltransferase, myeloid-specific|fucT-IV|fucosyltransferase IV|galactoside 3-L-fucosyltransferase|stage-specific embryonic 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FUT4, check out the FUT4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FUT4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated (FC04535-FITC)
Hello CJ!
FC04535-FITC has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Isolation, Culture and Biological Characteristics of Primordial Germ Cells from Beijing Fatty Chicken
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated
Question
I see that the anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-19
Question
Is a blocking peptide available for product anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated (FC04535-FITC)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We do provide the blocking peptide for product anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated (FC04535-FITC). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-31
Question
See below the WB image, lot number and protocol we used for testis using anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
Can you help my question with product FC04535-FITC, anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-07
Question
Is this FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated reactive to the isotypes of FUT4?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The immunogen of FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated is Purified human neutrophils. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-01
Question
I am interested in to test anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC on human testis for research purposes, then I may be interested in using anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Williams
Verified customer
Asked: 2019-09-23
Answer
The products we sell, including anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-23
Question
Will FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
You can see on the product datasheet, FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-26
Question
Do you have a BSA free version of anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC available?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-22
Question
Will anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC work for Flow Cytometry with testis?
Z. Carter
Verified customer
Asked: 2019-01-28
Answer
According to the expression profile of testis, FUT4 is highly expressed in testis. So, it is likely that anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC will work for Flow Cytometry with testis.
Boster Scientific Support
Answered: 2019-01-28
Question
I was wanting to use your anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated for Flow Cytometry for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
It shows on the product datasheet, FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC. Let me know if you need anything else.
K. Patel
Verified customer
Asked: 2017-09-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-26
