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Product Info Summary
| SKU: | M04535 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody
SKU/Catalog Number
M04535
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (Catalog # M04535). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04535)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: FUT4/815
Isotype
IgM, kappa
Immunogen
Purified human neutrophils
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M04535 is reactive to FUT4 in Human, Mouse, Rat
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
59084 MW
Background of SSEA-1
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04535 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Anti-CD15 Mouse Monoclonal Antibody (FUT4/815).
Protein Target Info & Infographic
Gene/Protein Information For FUT4 (Source: Uniprot.org, NCBI)
Gene Name
FUT4
Full Name
Alpha-(1,3)-fucosyltransferase 4
Weight
59084 MW
Superfamily
glycosyltransferase 10 family
Alternative Names
Alpha- (1,3)-fucosyltransferase 4;2.4.1.-;ELAM-1 ligand fucosyltransferase;Fucosyltransferase 4;Fucosyltransferase IV;Fuc-TIV;FucT-IV;Galactoside 3-L-fucosyltransferase;FUT4;ELFT, FCT3A; FUT4 CD15, ELFT, FCT3A, FUC-TIV, FUTIV, LeX, SSEA-1 fucosyltransferase 4 alpha-(1,3)-fucosyltransferase 4|ELAM ligand fucosyltransferase|ELAM-1 ligand fucosyltransferase|Lewis X|alpha (1,3) fucosyltransferase, myeloid-specific|fucT-IV|fucosyltransferase IV|galactoside 3-L-fucosyltransferase|stage-specific embryonic 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FUT4, check out the FUT4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FUT4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (M04535)
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No publications found for M04535
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody
Question
Will M04535 anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
As indicated on the product datasheet, M04535 anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-30
Question
Is this M04535 anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody reactive to the isotypes of FUT4?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The immunogen of M04535 anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody is Purified human neutrophils. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-27
Question
Does anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 work on bovine IHC with testis?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Our lab technicians have not tested anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
I was wanting to use your anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody for IHC for rat bone marrow on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat bone marrow identification?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
It shows on the product datasheet, M04535 anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
Does anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 work for IHC with bone marrow?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
According to the expression profile of bone marrow, FUT4 is highly expressed in bone marrow. So, it is likely that anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 will work for IHC with bone marrow.
Boster Scientific Support
Answered: 2019-10-28
Question
My colleagues were satisfied with the WB result of your anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody. However we have observed positive staining in bone marrow golgi stack membrane using this antibody. Is that expected? Could you tell me where is FUT4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
From literature, bone marrow does express FUT4. Generally FUT4 expresses in golgi apparatus, golgi stack membrane. Regarding which tissues have FUT4 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-08
Question
Is a blocking peptide available for product anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody (M04535)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
We do provide the blocking peptide for product anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody (M04535). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-13
Question
I am interested in to test anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 on rat bone marrow for research purposes, then I may be interested in using anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
The products we sell, including anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-19
Question
We are currently using anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
The anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody (M04535) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-18
Question
See below the WB image, lot number and protocol we used for bone marrow using anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-12
Question
I have a question about product M04535, anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04535 anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-06
Question
We have been able to see staining in human testis. Are there any suggestions? Is anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody supposed to stain testis positively?
L. Kulkarni
Verified customer
Asked: 2018-09-05
Answer
According to literature testis does express FUT4. According to Uniprot.org, FUT4 is expressed in bone marrow, testis, peripheral blood leukocyte, among other tissues. Regarding which tissues have FUT4 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-09-05
Question
Is there a BSA free version of anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 available?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-03
Question
I see that the anti-CD15 / FUT4 (Reed-Sternberg Cell Marker) Monoclonal antibody M04535 works with IHC, what is the protocol used to produce the result images on the product page?
D. Parker
Verified customer
Asked: 2017-03-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-03-30
