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Facts about Alpha-(1,3)-fucosyltransferase 4.
Human | |
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Gene Name: | FUT4 |
Uniprot: | P22083 |
Entrez: | 2526 |
Belongs to: |
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glycosyltransferase 10 family |
alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1
Mass (kDA):
59.084 kDA
Human | |
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Location: | 11q21 |
Sequence: | 11; NC_000011.10 (94543851..94549898) |
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi.
This article will show you how to utilize the FUT4 marker in a variety of biological applications. This article will explain how to use FUT4 or other molecular marker for different research purposes. These tips can help you maximize your research. Before you buy any of these kits, it's important to know your research goals.
Boster Bio FUT4 ELISA kits allow you to detect FUT4 presence in human samples. Its proprietary ELISA platform, PicoKine(tm), delivers high-sensitivity ELISA kits. The company's products are used in a wide range of research applications, including immunohistochemistry, Western Blotting, and ELISA. Here are some tips on optimizing your experiment using the Boster BIO FUT4 ELISA set.
This ELISA kit detects FUT4 from serum. It uses a synthetic peptide that corresponds to the human FUT4 sequence. The reagent may be kept at room temperature for up to one year or frozen at room temperature for six months. It contains Trehalose and blocking progesterone. Follow the instructions on your package to test your samples.
The Boster Bio FUT4 ELISA is an easy-to-use and reliable method for the detection of FUT4 levels in blood samples. The kit uses three reagents that can be used in tandem. First, purchase Anti-miR200c. This antibody targets the 3'-UTR of mRNA. Anti-miR200c mimics inhibit FUT4's biosynthesis, while FUT4 siRNA increases its expression.
Boster Bio FUT4 ELISA not only measures this antibody but also measures miR-220c. According to the results of miR200c, FUT4 levels were correlated with this antibody. A receiver operating characteristic curve was also created to evaluate the test's sensitiveness. These results can then be used to determine if Boster Bio ELISA for FUT4 is the right test. This kit has many advantages.
The key step in developing therapeutic antibodies is to produce a high-affinity primary antibody using the FUT4 marker. Without this protein, antibodies can be redirected towards non-specific targets. This could lead to an autoimmune disorder, delayed transition from primary autoreactive to secondary antigen-specific antibody reactions, or even a delayed transition. However, this technique requires careful planning and execution.
The IgD-class IgD BCR controls antibody release in vivo. It also senses antigen-valence. Monovalent antigens block the production IgG antibodies. This results in the activation high-affinity IgM. Polyvalent antigens stimulate IgG antibody production and activate mature B cells to produce IgG antibodies. Because of its important role in B cell activation and tolerance to self-structures, the FUT4 marker has gained increasing attention in immunotherapy.
IgD regulates autoreactive IgG response duration. IgD-deficient antibodies have a decreased memory immune response. Autoimmune diseases that result in the development of autoreactive IgG antibody antibodies require the generation and maintenance of long-lasting antibody memories. In addition, IgD deficiency can inhibit the activity and secretion of antibodies-secreting cells. The IgD-class BCR is also less expressed in antibody secreting cells. This suggests that they are more prone to initiating rapid primary immune responses.
A polyclonal antibody is a monoclonal, specialized antibody that binds only to the epitopes a specific antigen. While antibodies can bind to a specific epitope of an antigen, they can also cross-react with other antigen proteins that may obscure the detection of the target antibody. These antibodies are removed by elution via a column containing an immobilized Antigen. This process removes the non-specific antibodies and leaves only the antigen-specific polyclonal antibody in the column.
The IHC optimized polyclonase resulted was validated to detect FUT4 in human skin biopsy samples. The sections were stained with an antibody against HDAC4 from rabbits and with a negative control. The sections were mounted under coverslips and incubated for 45 min. The sections were stained brown due to the precipitated DAB reaction product resulting from the HDAC4 antigen. The corresponding negative control section showed only a blue hematoxylin contrastain.
A number of suppliers offer anti FUT4 monoclonal antigen antibodies. This antibody recognizes FUT4 protein (also known under the names Lewis X, ELFT and EFLT). All steps must always be optimized in order for IHC/ICC experiments to be successful. This will allow you to see specific staining while minimizing background signals. Primary antibodies must be tested in order to select them. A working dilution of monoclonal antibody is lower than one of polyclonal. A high-quality antibody will exhibit minimal cross-reactivity.
The Boster Bio Monoclonal Antibody FUT4 / Anti-CD15 / FUT4 reacts with Human, Mouse, Rat cells. It has been verified by immunofluorescence. This monoclonal antibody is suitable for all kinds of research work, including immunohistochemistry, cell culture and genetics. Scientists worldwide can benefit from its use for various applications and species-specific studies.
Boster Bio's antibody library contains over 12,000 antibodies. They are validated in WB and Flow as well as IHC. The Boster Bio antibodies are quantitatively tested against a panel consisting of 250 tissue samples. They are also validated against untransfected cell lines. Boster Bio FUT4Marker antibody is highly sensitive. It has a high affinity to the recombinant Protein.
PMID: 1702034 by Goelz S.E., et al. ELFT: a gene that directs the expression of an ELAM-1 ligand.
PMID: 1718983 by Kumar R., et al. Cloning of a human alpha(1,3)-fucosyltransferase gene that encodes ELFT but does not confer ELAM-1 recognition on Chinese hamster ovary cell transfectants.
*More publications can be found for each product on its corresponding product page