Click image to see more details
Product Info Summary
| SKU: | M00652-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD62L Purified SELL Monoclonal Antibody
View all L-Selectin/CD62L Antibodies
SKU/Catalog Number
M00652-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD62L Purified SELL Monoclonal Antibody (Catalog# M00652-2). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD62L Purified SELL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00652-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
DREG56
Isotype
Mouse IgG1
Immunogen
PMA-activated human peripheral blood leukocytes. The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00652-2 is reactive to SELL in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
42187 MW
Background of L-Selectin/CD62L
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00652-2 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml.
Western blotting: Non-reducing conditions.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry validation of SELL using Anti-Hu CD62L Purified SELL Monoclonal Antibody (M00652-2).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 μg/ml) GAM APC.
Click image to see more details
Figure 2. Flow Cytometry validation of SELL using Anti-Hu CD62L Purified SELL Monoclonal Antibody (M00652-2).
Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 μg/ml) GAM APC.Protein Target Info & Infographic
Gene/Protein Information For SELL (Source: Uniprot.org, NCBI)
Gene Name
SELL
Full Name
L-selectin
Weight
42187 MW
Superfamily
selectin/LECAM family
Alternative Names
L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 -like family member L|gp90-MEL|leukocyte surface Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SELL, check out the SELL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SELL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD62L Purified SELL Monoclonal Antibody (M00652-2)
Hello CJ!
No publications found for M00652-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD62L Purified SELL Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Hu CD62L Purified SELL Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
