L-selectin Antibodies

L-selectin (SELL)

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28489325, PubMed:28011641). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes.

Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641). .

Gene Information

Human
Gene Name:	SELL
Uniprot:	P14151
Entrez:	6402

Family

Belongs to:
selectin/LECAM family

Alternative Names

CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

Molecular Weight

Mass (kDA):
42.187 kDA

Genomic Context

Human
Location:	1q24.2
Sequence:	1; NC_000001.11 (169690667..169711620, complement)

Tissue Specificity

Expressed in B-cell lines and T-lymphocytes.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein.

Diseases

• Malignant Neoplasms
• Tissue Adhesions
• Mental Disorders
• Inflammation
• Neoplasms
• Atherosclerosis
• Spinal Cord Injuries
• Carcinoma
• Lymphoma
• Inflammatory Response
• Streptococcal Infections
• Psychotic Disorders
• Spinal Injuries

• Liver Carcinoma
• Malignant Paraganglionic Neoplasm
• Infective Disorder
• Malignant Neoplasm Of Prostate
• Hyperplasia
• Malignant Neoplasm Of Breast
• 5,10-methylenetetrahydrofolate Reductase Deficiency

Pathways

• Pathogenesis
• Immune Response
• Cell Activation
• Inflammatory Response
• Cell Adhesion
• Aging
• Proteolysis
• Innate Immune Response
• Cell Proliferation
• Endothelial Cell Activation
• Cell Growth
• Transport
• Diapedesis

• Neutrophil Extravasation
• Hypersensitivity
• Reverse Transcription
• Cell Cycle
• Leukocyte Homeostasis
• Cytolysis
• Membrane Fusion

PTMs

• Phosphorylation
• Glycosylation

• Cleavage
• Methylation

• Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SELL

Best Uses Of The SELL Marker

There are a variety of ways to optimize your experiments with the Boster Bio Super Vision detection kits. Using Boster Bio's primary-secondary-ABC system will help you locate proteins of interest with low background and high specificity. Check out the following article for more details. Here are some tips on how to prepare your samples for Boster Bio's SELL Marker. Once your samples are ready and ready to go, you can begin the experiments with the Boster Bio Super Vision detection system.

Boster Bio's primary-secondary-ABC system allows researchers to locate the proteins of interest

With its own technology platform that is scalable, Boster Biologicals manufactures a broad range of biomarkers and targets that are druggable for immunological as well as cell-based research. This technology has numerous applications for human disease, such as research into cell therapies and drug discovery. Boster Bio's antibodies and enzyme kits identify biomarkers for inflammation and cancer. Boster Bio's PICOKINE (tm) ELISA kit has a picogram-level sensitivity making it a good option for various types of research.

The system can also help researchers identify the proteins of interest, including the ones that are involved in immunotherapies based on cancer cells. The primary-secondary-ABC system of Boster Bio's antibodies has a wide range of applications, from identifying protein targets to analyzing their molecular function. Its antibodies are suitable for studies on epigenetics and post-translational modifications.

MagnaLINK(r), streptavidin magnetic beads have fifteen times more effectiveness in binding biotin than commercially available products. The ChromaLINK(r), Biotin, and Digoxigenin products allow for the precise incorporation of labels biological molecules without the need for lengthy tests. Boster Bio is a global leader in Life Science Research and also offers transfection tools, reagents for viral research, and other support services to Eukaryotes cell-based research.

The primary-secondary-ABC system uses avidins conjugated with a signal molecule - usually Horseradish Peroxide -- to target the proteins of interest in biological samples. The primary-secondary-ABC system has low background and high specificity, making it a versatile option for researchers. Other detection systems utilize organic polymers and polysaccharides.

Boster Bio's Super Vision Detection Kits allow researchers to identify proteins of interest with high specificity and with low background.

The Super Vision Detectaions Kits made by Boster Bio are extremely sensitive and are designed to detect tiny amounts of protein in biological samples. The Super Vision Detectaions Kits can be used with a variety analytical techniques including IHC, WB and ELISA. Researchers can find the proteins that are of interest quickly and easily with low background and high specificity.

This RT-PCR Kit is highly sensitive and easy to use. It uses the qScript cDNA synthesizer kit. It contains all the necessary components to enable cDNA synthesis, and also features a ribonuclease inhibit protein. The Reaction Mix contains all the necessary components to cDNA synthesizing, including an engineered MMLV reverse transcriptionase (RT) and the ribonuclease inhibitor (RIPA) protein. The qScript Kit for cDNA Synthesis is designed to be highly sensitive and durable to avoid amplifying target proteins through repeatedly freezing and thawing.

The primary-secondary-ABC detection system involves avidins conjugated to signal molecules, such as Horseradish Peroxide (HRP), to allow researchers to detect the proteins of interest. It is the most sensitive method of detection for small amounts of protein . It also has high specificity and low background. Other detection systems use organic polymers and polysaccharides in order to detect small amounts of protein.

The steps involved in acquiring testing kits, performing the test and showing the results vary for each kit. You can purchase the Everlywell COVID-19 Test Home Collection Kit over-the counter at local pharmacies or from online retailers. After purchasing the kit users will need to complete an intake questionnaire as well as provide a nasal sample and then collect saliva samples. Then, the sample will be shipped to a CLIA-certified laboratory for testing using RT-PCR.

Boster Bio's Sell Marker

Boster Bio has become an industry leader in the creation of high-specific and high-sensitivity antibodies for different applications. It was founded by Steven Xia in 1993, the company has spent the past two decades perfecting its techniques and methods. Their products are referenced in more than 29,000 scientific papers. The antibodies are standardized to meet the requirements of several tests for immunoassays, such as IHC, WB, and ELISA.

Sample preparation

The preparation of the sample for the SELLMarker is essential for ensuring high quality analysis. Data correction routines can't correct mistakes made at the beginning of the process. When handling can introduce foreign material into the specimen, polishing abrasives can cause it to be absorbed into the specimen. The algorithms that correct the errors will have to be able identify the geometry of the specimen. It is not that difficult to prepare samples for the SELL Marker, as it might seem.

Troubleshooting

A successful troubleshooting process is to bring the system back to its previous condition. This usually indicates that the issue was resolved and the functions of the product have been restored. The experience of the technician and their thoroughness will determine the effectiveness of troubleshooting. To avoid negative consequences the procedure should be closely monitored. It is important to keep in mind that both technical expertise and the root cause are essential to identifying the issue.

One approach to troubleshoot the SELL Marker is to employ an approach that is tiered. The troubleshooter begins by reviewing the system at a high level and then goes through each component until the issue is resolved. Once the issue is solved the troubleshooter re-runs the procedure in the affected area and saves notes in a checklist.