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Product Info Summary
| SKU: | PB9389 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CD62L/SELL Antibody Picoband®
View all L-Selectin/CD62L Antibodies
SKU/Catalog Number
PB9389
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD62L/SELL Antibody Picoband® catalog # PB9389. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD62L/SELL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9389)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human L-selectin recombinant protein (Position: W39-Y372). Human L-selectin shares 77.5 % and 78.7 % amino acid (aa) sequence identity with mouse and rat L-selectin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9389 is reactive to SELL in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42187 MW
Background of L-Selectin/CD62L
CD62L, also known as L-selectin, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9389 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Human Placenta Tissue, JURKAT Whole Cell, CEM Whole Cell, HL60 Whole Cell, Rat Spleen Tissue, Mouse Spleen Tissue
IHC: Human Tonsil Tissue
Validation Images & Assay Conditions
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Anti-L-selectin Picoband antibody, PB9389, IHC(P)
IHC(P): Human Tonsil Tissue
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Anti-L-selectin Picoband antibody, PB9389, Western blotting
All lanes: Anti L-selectin (PB9389) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: HL60 Whole Cell Lysate at 40ug
Lane 5: Rat Spleen Tissue Lysate at 50ug
Lane 6: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 42KD
Observed bind size: 42KD
Protein Target Info & Infographic
Gene/Protein Information For SELL (Source: Uniprot.org, NCBI)
Gene Name
SELL
Full Name
L-selectin
Weight
42187 MW
Superfamily
selectin/LECAM family
Alternative Names
L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte surface antigen Leu-8;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;TQ1;gp90-MEL;CD62L;SELL;LNHR, LYAM1; SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 -like family member L|gp90-MEL|leukocyte surface Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SELL, check out the SELL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SELL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD62L/SELL Antibody Picoband® (PB9389)
Hello CJ!
PB9389 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pearse DD, Anthony Otero P, Diaz A, Pan X, Ghosh M. Neuronal and endothelial transglutaminase-2 expression during experimental autoimmune encephalomyelitis and multiple sclerosis. Neuroscience. 2020 Nov 27:S0306-4522(20)30756-9. doi: 10.1016/j.neuroscienc
Species: Human,Mouse,Rat
PB9389 usage in article: APP:IHC, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:100
HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes
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Customer Q&As
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1 Customer Q&As for Anti-CD62L/SELL Antibody Picoband®
Question
We are currently using anti-CD62L/SELL antibody PB9389 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The anti-CD62L/SELL antibody (PB9389) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-04
