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Product Info Summary
| SKU: | EK0937 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat sL-Selectin ELISA Kit PicoKine®
View all L-Selectin/CD62L ELISA kits
SKU/Catalog Number
EK0937
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat sL-Selectin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat SELL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat sL-Selectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0937)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: W39-N332
Sensitivity
<300 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0937 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0937 is reactive to SELL in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0937 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of L-Selectin/CD62L
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0937-CAP | Anti-Rat SELL Pre-coated 96-well strip microplate | 1 |
| EK0937-ST | Rat SELL Standard | 2 vials, 40 ng/tube |
| EK0937-DA | Rat SELL Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 625 | 2000000 | 4000000 | 200000 | 400000 | 20000 | 40000 |
| O.D. | 0.004 | 0.550 | 0.919 | 1.411 | 1.799 | 2.074 | 2.129 | 2.226 |
Data Example Images
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Rat sL-Selectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat sL-Selectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1474 | 3614 | 24600 | 1588 | 3716 | 23760 |
| Standard deviation | 89.91 | 166.24 | 1279.2 | 100.04 | 178.36 | 1663.2 |
| CV (%) | 6.1% | 4.6% | 5.2% | 4.8%% | 4.8% | 7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of L-Selectin/CD62L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1474 | 1304 | 1342 | 1320 | 1360 | 67.18 | 4.9% |
| Sample 2 | 3614 | 3452 | 3351 | 3314 | 3432 | 116.19 | 3.3% |
| Sample 3 | 24600 | 22997 | 23584 | 22075 | 23314 | 916.82 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For SELL (Source: Uniprot.Org, NCBI)
Gene Name
SELL
Full Name
L-selectin
Weight
42471 MW
Superfamily
selectin/LECAM family
Alternative Names
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1 SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 antigen-like family member L|gp90-MEL|leukocyte surface antigen Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SELL, check out the SELL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SELL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat sL-Selectin ELISA Kit PicoKine® (EK0937)
Hello CJ!
EK0937 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of hypercapnia on T cells in lung ischemia/reperfusion injury after lung transplantation:
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Rat sL-Selectin ELISA Kit PicoKine®
Question
Q: Can CD62L ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-12-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-12-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-11-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-11-06
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD62L ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD62L for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-06-27
Question
Q: how to analyze ELISA data? I measured CD62L level in cell lysates.
Verified Customer
Verified customer
Asked: 2019-06-15
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-15
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
R. Baker
Verified customer
Asked: 2019-05-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-05-20
