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Product Info Summary
| SKU: | M03484-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P |
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Product info
Product Name
Anti-Hu CD52 Purified Monoclonal Antibody
SKU/Catalog Number
M03484-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD52 Purified Monoclonal Antibody (Catalog# M03484-1). Tested in Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD52 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03484-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
HI186
Isotype
Mouse IgG2b
Immunogen
Human tonsil. The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03484-1 is reactive to CD52 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
6614 MW
Background of CD52
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03484-1 is guaranteed for Flow Cytometry, IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of CD52 using Anti-Hu CD52 Purified Monoclonal Antibody (M03484-1).
Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE).1A – CD52 negative pro T ALL (gate leukemic cells)
1B – CD52 positive cALL (gate leukemic cells)
Protein Target Info & Infographic
Gene/Protein Information For CD52 (Source: Uniprot.org, NCBI)
Gene Name
CD52
Full Name
CAMPATH-1 antigen
Weight
6614 MW
Alternative Names
CAMPATH-1, HE5, ESPE5 CD52 CDW52, EDDM5, HE5 CD52 molecule CAMPATH-1 |CD52 (CAMPATH-1 )|CDW52 (CAMPATH-1 )|HEL-S-171mP|cambridge pathology 1 |epididymal secretory protein E5|epididymis secretory sperm binding protein Li 171mP|human epididymis-specific protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD52, check out the CD52 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD52: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD52 Purified Monoclonal Antibody (M03484-1)
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