Product Info Summary
SKU: | A02284-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-Sp2 (D512) Antibody
SKU/Catalog Number
A02284-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Sp2 (D512) Antibody catalog # A02284-1. Tested in IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sp2 (D512) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02284-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human AT1A3 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02284-1 is reactive to SP2 in Human, Mouse, Rat
Applications
A02284-1 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
64900 MW
Background of SP2
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Lawrance S.K., Nucleic Acids Res. 13:7515-7528(1985).
Gustafsson K., J. Biol. Chem. 262:8778-8786(1987).
Young J.A., Hum. Immunol. 23:37-44(1988).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 1:50-1:200
Validation Images & Assay Conditions
![a02284 1 anti vinculin vcl antibody biotin ihc testing 1 a02284 1 anti vinculin vcl antibody biotin ihc testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a02284-1-anti-vinculin-vcl-antibody-biotin-ihc-testing-1.jpg)
Click image to see more details
Figure 1. Immunohistochemistry validation of SP2 using Anti-Sp2 (D512) Antibody (A02284-1).
Immunohistochemistry (IHC) analyzes of Sp2 (D512) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibodyProtein Target Info & Infographic
Gene/Protein Information For SP2 (Source: Uniprot.org, NCBI)
Gene Name
SP2
Full Name
Transcription factor Sp2
Weight
64900 MW
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
KIAA0048; Sp2 transcription factor; transcription factor Sp2 SP2 Sp2 transcription factor transcription factor Sp2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SP2, check out the SP2 Infographic
![SP2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sp2 (D512) Antibody (A02284-1)
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