Click image to see more details
Product Info Summary
| SKU: | FC01340-PE-Cy7 |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated
SKU/Catalog Number
FC01340-PE-Cy7
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated (Catalog# FC01340-PE-Cy7). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01340-PE-Cy7)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: L243
Isotype
IgG2a,k
Immunogen
Human B lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01340-PE-Cy7 is reactive to CD74 in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
33516 MW
Background of CD74
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01340-PE-Cy7 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Monocytes gated PBMCs stained with PE-Cyanine7 conjugated anti-human HLA-DR (clone L243) (red histogram). Monocytes gated PBMCs stained with PE-Cyanine 7 conjugated mouse IgG2a isotype control (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD74 (Source: Uniprot.org, NCBI)
Gene Name
CD74
Full Name
HLA class II histocompatibility antigen gamma chain
Weight
33516 MW
Alternative Names
HLA class II histocompatibility antigen gamma chain;HLA-DR antigens-associated invariant chain;Ia antigen-associated invariant chain;Ii;p33;CD74;CD74;DHLAG; CD74 DHLAG, HLADG, II, Ia-GAMMA, p33 CD74 molecule HLA class II histocompatibility gamma chain|CD74 (invariant polypeptide of major histocompatibility complex, class II -associated)|CD74 molecule, major histocompatibility complex, class II invariant chain|HLA-DR s-associated invariant chain|HLA-DR-gamma|Ia-associated invariant chain|MHC HLA-DR gamma chain|gamma chain of class II s
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD74, check out the CD74 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD74: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated (FC01340-PE-Cy7)
Hello CJ!
No publications found for FC01340-PE-Cy7
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated
Question
Do you have a BSA free version of anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated FC01340-PE-Cy7 available?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated FC01340-PE-Cy7 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell tonsil using anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated FC01340-PE-Cy7. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-15
Question
My question regarding product FC01340-PE-Cy7, anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01340-PE-Cy7 anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-10
Question
I was wanting to use your anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated for Flow Cytometry for human b-cell tonsil on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human b-cell tonsil identification?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
It shows on the product datasheet, FC01340-PE-Cy7 anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell tonsil in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
