CAMPATH-1 antigen Antibodies

CAMPATH-1 antigen (CD52)

May play a role in carrying and orienting carbohydrate, in addition to having a more specific function. .

Gene Information

Human
Gene Name:	CD52
Uniprot:	P31358
Entrez:	1043

Family

Belongs to:
No superfamily

Alternative Names

Cambridge pathology 1 antigen; CAMPATH-1 antigen; CAMPATH-1; CD52 antigen (CAMPATH-1 antigen); CD52 antigen; CD52 molecule; CD52; CDW52 antigen (CAMPATH-1 antigen); CDw52; Epididymal secretory protein E5; He5; HEL-S-171mP; Human epididymis-specific protein 5

Molecular Weight

Mass (kDA):
6.614 kDA

Genomic Context

Human
Location:	1p36.11
Sequence:	1; NC_000001.11 (26317958..26320523)

Subcellular Localizations

Cell membrane; Lipid-anchor, GPI-anchor.

Diseases

• Leukemia
• Chronic Lymphocytic Leukemia
• Lymphoid Leukemia
• Lymphoma
• Malignant Neoplasms
• Neoplasms
• Graft-vs-host Disease
• Decreased Immunologic Activity [pe]
• Infective Disorder
• Malignant Paraganglionic Neoplasm
• T-cell Lymphoma
• Autoimmune Diseases
• Autoimmune Reaction

• B-cell Lymphomas
• Hematologic Neoplasms
• Lymphoma, Non-hodgkin
• Infertility
• Acute Lymphocytic Leukemia
• Prolymphocytic Leukemia
• Sclerosis

Pathways

• Pathogenesis
• Antibody-dependent Cellular Cytotoxicity
• Localization
• Cell Death
• Cell Proliferation
• Complement-dependent Cytotoxicity
• Glycosylation
• T Cell Proliferation
• Secretion
• Fertilization
• Cell Activation
• T Cell Activation
• Cell Differentiation

• Immune Response
• Complement Activation
• Phagocytosis
• Leukocyte Differentiation
• Ovulation
• Opsonization
• Transport

PTMs

• Gpi Anchoring
• Glycosylation
• Phosphorylation

• Cleavage
• Acylation
• Palmitoylation

• Deglycosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CD52

Best Uses Of The CD52 Marker

Scientists may want to know how to identify CD8 cells using the CD52 marker. WB/IHC, ELISA, and WB/IHC are both popular methods. However you may be curious as to how to use the CD52 mark. The following article provides an overview of how these two markers are used, as well as their best applications. Below are some tips to help you perform these tests.

CD52

Boster Bio: CD52 is a small glycosylated peptide bound to the cell membrane through a GPI link. It is found in T and B cells, as well other cell types like monocytes, macrophages and eosinophils. It is believed to be a co-stimulatory molecule that inhibits T cell activation and induces CD4+ regulatory T cells.

M13

The CD52 marker, a small glycosylated progesterone, is attached to the cell membrane via a GPI connection. This protein is expressed in human lymphocytes, male reproductive tissue, and mature sperm cell lines. Alemtuzumab is a humanized monoclonal antibody that targets CD52. It is used in lymphoma treatment and to deplete T cell levels. It has been shown also to induce adaptive Tregs. These are immune cells that have not been activated. A phospholipase enzyme is responsible for cleaving the CD52 marker. This enzyme prefers to bind normal CD52 to CD52 antibodies.

Certain autoimmune diseases such as Lupus can be detected by using the CD52 marker. Patients who have recovered from autoimmune diseases tend to decrease this marker. In addition to treating autoimmune diseases, researchers are testing CD52 marker testing for the early detection of macular degeneration. Furthermore, researchers are developing therapies to treat multiple sclerosis and Alzheimer's disease with this marker.

The CD52 molecule is a dual function protein. It serves two functions: a costimulator as well as a surface marker. The primary structure of CD52 is Treumann A, which is responsible for promoting the production of CD4+ regulatory T cells. CD52 stimulates the formation and suppression of regulatory T cells. CD52 is also a binding molecule for T cell receptors. These receptors regulate immune reactions and protect against infections.

ELISA

ELISAs, which measure the amount a specific antigen on a cell's surface, are highly sensitive and specific tests. These assays are generally performed using immunofluorescence techniques and enzyme-linked immunosorbant assays. These techniques utilize the special properties of an antigen-antibody system as well as simple phase separations, to produce highly sensitive or specific assays. Boster Bio antibody are suitable for use in IHC (WB), ELISA (ELISA), and Flow Cytometry.

Boster Bio has used a synthetic CD52 marker in this ELISA. The test uses a test where the target cell expresses CD52. Serial dilution of known quantities of CD52 molecules that have reached saturation in Campath-1H creates the standard curve. This method can be used to detect a wide range of cells, such as T-cell malignancies or myeloid leukemia.

The ELISA also detected immune complicatedes in CLL patients' samples. The immune complexes were formed during the immune responses and reduced the antibody's ability to attach to the target cell. The findings suggest that the formation of immune complexes inhibits the activity of the targeted cell-specific antibody, which may lead to a shortened life expectancy.

Another advantage to this ELISA is its ability to measure the levels in blood samples of soluble leukocyte-surface molecules. This marker is associated in CLL patients with different stages such as lymph node involvement and CD52 count. The sCD52 marker can be used to help patients be staged and monitor the effectiveness of therapy. Although it is difficult to determine the exact role of CD52 in patients, it is still a helpful tool in monitoring disease activity and therapy response.

WB/IHC

Boster Bio produces high-sensitivity antibodies that are specific and sensitive for use in a variety if applications. The company was founded by Boster Bio in 1993. They have spent more than 20 years developing their technology. Their antibodies have been cited by over 29,000 publications. They are approved for use in Flow Cytometry and ELISA as well as IHC. This article explains the most popular uses of this marker as well as how to make the most out of this protein in research.

Applications

The CD52 marker, a protein expressed on the surfaces of various cell types, is known as a protein. Its peptide-backbone is encoded in HE5 mRNA. This mRNA can be found in the distal corpus epididymis. There are several suppliers of antibodies against CD52. Each antibody has its specificity and immunogen. Here are some of these applications for the CD52 marker.

The TCGA database reported that CD52 levels are significantly higher in BC samples than in paired controls. This marker has been proven to predict prognosis in BC patients. This marker could potentially be used in BC as a prognosticator and a therapeutic target. Further research is needed to determine its role in cancer. This marker could be used to develop treatments for cancer in the future.

The CD52 marker can be used to monitor the patient's reaction to treatment. The CD52 marker, which is used in Lupus, measures the patient's reaction to the drug. The CD52 level decreases slowly in autoimmune diseases. Researchers are also testing CD52 for markers to detect early signs in macular and multiple sclerosis. The CD52 marker can be used for diagnostic purposes.

The monoclonal antibody CF1D12 reacts against human CD52. This antibody recognizes both the CD52 carbohydrate area and the CD52 protein core. This clone has been used in flowcytometry and Western blot. The monoclonal immunoglobulin reacts with CD52 glycoforms, which are human sperm glycoforms, in western-blot. These results support that CD52 is being shed.