Anti-HSD17B2 Antibody Picoband®

Product Name

Anti-HSD17B2 Antibody Picoband®

View all HSD17B2 Antibodies

SKU/Catalog Number

PA1604

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSD17B2 Antibody catalog # PA1604. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSD17B2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1604)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1604 is reactive to HSD17B2 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

43 kDa

Calculated molecular weight

42785 MW

Background of HSD17B2

HSD17B2 (17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42 (43%) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1604 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: Human Placenta Tissue
IHC: Human Placenta Tissue
IHC-F: Human Placenta Tissue

Validation Images & Assay Conditions

Gene/Protein Information For HSD17B2 (Source: Uniprot.org, NCBI)

Alternative Names

Estradiol 17-beta-dehydrogenase 2;1.1.1.62;17-beta-hydroxysteroid dehydrogenase type 2;17-beta-HSD 2;20 alpha-hydroxysteroid dehydrogenase;20-alpha-HSD;E2DH;Microsomal 17-beta-hydroxysteroid dehydrogenase;Short chain dehydrogenase/reductase family 9C member 2;Testosterone 17-beta-dehydrogenase;1.1.1.239;HSD17B2;EDH17B2, SDR9C2; HSD17B2 EDH17B2, HSD17, SDR9C2 hydroxysteroid 17-beta dehydrogenase 2 17-beta-hydroxysteroid dehydrogenase type 2|17-beta-HSD 2|20 alpha-hydroxysteroid dehydrogenase|20-alpha-HSD|E2DH|estradiol 17-beta-dehydrogenase 2|microsomal 17-beta-hydroxysteroid dehydrogenase|short chain dehydrogenase/reductase family 9C member 2|testosterone 17-beta-dehydrogenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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