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Product Info Summary
| SKU: | EK7105 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | Cell Lysates, Tissue, Serum |
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Product info
Product Name
Alpha B Crystallin ELISA Kit
See all AlphaB Crystallin/CRYAB products
SKU/Catalog Number
EK7105
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Colorimetric detection of Alpha B Crystallin. 96 wells/kit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Alpha B Crystallin ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7105)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.009 ng/ml
Assay Range
0.352 - 22.5 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7105 is reactive to CRYAB in Human samples
Validated Sample Types
Cell Lysates, Tissue, Serum
Application Guarantee
EK7105 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Anti-Alpha B Crystallin Immunoassay Plate | 1 Plate |
| 5X Alpha B Crystallin Extraction Reagent | 1 vial/10 ml |
| Recombinant Alpha B Crystallin Standard | 2 vials |
| Standard and Sample Diluent | 1 vial/ 50 ml |
| 10X Wash Buffer Concentrate | 1 vial/100 ml |
| Anti-Alpha B Crystallin Biotinylated Antibody Concentrate | 1 vial/150 µl |
| Anti-Alpha B Crystallin Biotinylated Antibody Diluent | 1 vial/ 13 ml |
| Streptavidin: HRP Concentrate | 1 vial/150 µl |
| Streptavidin: HRP Diluent | 1 vial/ 13 ml |
| TMB Substrate | 1 vial/ 13 ml |
| Stop Solution | 1 vial/ 13 ml |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Typical Standard Curve for the Alpha B Crystallin ELISA Kit (Enzyme-Linked Immunosorbent Assay) -EK7105.
Assay Type: Sandwich ELISA.
Detection Method: Colorimetric Assay.
Assay Range: 0.352 -22.5 ng/mL.
Specific Publications For Alpha B Crystallin ELISA Kit (EK7105)
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Customer Q&As
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2 Customer Q&As for Alpha B Crystallin ELISA Kit
Question
Hello Boster; I used your product Alpha B Crystallin ELISA Kit in my EMSA experiment and found a band. Since It is a polyclonal antibody (whole antiserum), I want to ask whether it is a purified or unpurified antibody? Also what kind of purification method?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The Alpha B Crystallin ELISA Kit has been no additional purification been done other than clotting being removed from whole blood (standard serum processing).
Boster Scientific Support
Answered: 2020-03-03
Question
I just want to inquire if this Alpha B Crystallin ELISA Kit has been tested in IHC, was it also tested with formalin fixed paraffin embedded sections, frozen sections, or both?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
Yes, the Alpha B Crystallin ELISA Kit has been tested for application in IHC-frozen sections but has not been tested in IHC-formalin fixed paraffin embedded sections.
Boster Scientific Support
Answered: 2018-06-22
