Anti-Heparanase 1/HPSE Antibody Picoband®

Product Name

Anti-Heparanase 1/HPSE Antibody Picoband®

View all Heparanase/HPSE Antibodies

SKU/Catalog Number

PB9427

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Heparanase 1/HPSE Antibody Picoband® catalog # PB9427. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Heparanase 1/HPSE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9427)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Heparanase 1, different from the related mouse and rat sequences by eight amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9427 is reactive to HPSE in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

61 kDa

Calculated molecular weight

61149 MW

Background of Heparanase/HPSE

Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9427 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: Rat Liver Tissue, Human Placenta Tissue, A549 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For HPSE (Source: Uniprot.org, NCBI)

Alternative Names

Heparanase;3.2.1.166;Endo-glucoronidase;Heparanase-1;Hpa1;Heparanase 8 kDa subunit;Heparanase 50 kDa subunit;HPSE;HEP, HPA, HPA1, HPR1, HPSE1, HSE1; HPSE HPA, HPA1, HPR11, HSE1, HPSE heparanase heparanase|endo-glucoronidase|heparanase-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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